Immunohistochemical staining of liver grafts with a monoclonal antibody against HCV-envelope 2 for recurrent hepatitis C after living donor liver transplantation

Hiroshi Sadamori, Takahito Yagi, Hiromi Iwagaki, Hiroaki Matsuda, Susumu Shinoura, Yuzo Umeda, Nobuya Ohara, Hiroyuki Yanai, Tetsuya Ogino, Noriaki Tanaka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Aim: We evaluated the expression of hepatitis C virus (HCV) antigen on liver grafts by immunohistochemical staining (IHS) using IG222 monoclonal antibody (mAb) against HCV-envelope 2 (E2). Methods: The study material was 84 liver biopsy specimens obtained from 28 patients who underwent living donor liver transplantation (LDLT) for HCV infection. The biopsy samples were examined histopathologically, and by IHS using IG222 mAb against HCV-E2. Serum HCV-RNA level was measured in all patients. The IHS grades were compared among the three groups classified according to the time elapsed from LDLT (at 1-30, 31-179 and ≥180 days post-LDLT) and among four post-transplant conditions, including acute cellular rejection (ACR). Results: Immunoreactivity to IG222 was detected in 78.6% of the specimens obtained during the first month after LDLT, and there were no significant differences on the IHS grades between the three groups classified according to the time elapsed from LDLT. The IHS grades were significantly stronger in definite recurrent HCV (n = 12) and probable recurrent HCV (n = 7) than in definite ACR (n = 7) and other complications (n = 8). There were no significant differences in serum HCV-RNA levels among the four post-transplant conditions. There was no significant correlation between the IHS grades using IG222 mAb and serum HCV-RNA levels when data of 84 liver biopsy specimens were analyzed. Conclusions: Constant HCV-E2 expression was observed in liver biopsy specimens obtained 1 month or longer. The strong HCV-E2 expression on liver grafts were associated with recurrent hepatitis C after LDLT, but the serum HCV-RNA levels were not.

Original languageEnglish
Pages (from-to)574-580
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume24
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

Living Donors
Hepatitis C
Hepacivirus
Liver Transplantation
Monoclonal Antibodies
Staining and Labeling
Transplants
Liver
RNA
Biopsy
Serum
Hepatitis C Antigens
Virus Diseases

Keywords

  • Acute rejection
  • Hepatitis C virus
  • Immunohistochemical staining
  • Liver transplantation
  • Recurrent hepatitis C

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Immunohistochemical staining of liver grafts with a monoclonal antibody against HCV-envelope 2 for recurrent hepatitis C after living donor liver transplantation. / Sadamori, Hiroshi; Yagi, Takahito; Iwagaki, Hiromi; Matsuda, Hiroaki; Shinoura, Susumu; Umeda, Yuzo; Ohara, Nobuya; Yanai, Hiroyuki; Ogino, Tetsuya; Tanaka, Noriaki.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 24, No. 4, 2009, p. 574-580.

Research output: Contribution to journalArticle

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abstract = "Aim: We evaluated the expression of hepatitis C virus (HCV) antigen on liver grafts by immunohistochemical staining (IHS) using IG222 monoclonal antibody (mAb) against HCV-envelope 2 (E2). Methods: The study material was 84 liver biopsy specimens obtained from 28 patients who underwent living donor liver transplantation (LDLT) for HCV infection. The biopsy samples were examined histopathologically, and by IHS using IG222 mAb against HCV-E2. Serum HCV-RNA level was measured in all patients. The IHS grades were compared among the three groups classified according to the time elapsed from LDLT (at 1-30, 31-179 and ≥180 days post-LDLT) and among four post-transplant conditions, including acute cellular rejection (ACR). Results: Immunoreactivity to IG222 was detected in 78.6{\%} of the specimens obtained during the first month after LDLT, and there were no significant differences on the IHS grades between the three groups classified according to the time elapsed from LDLT. The IHS grades were significantly stronger in definite recurrent HCV (n = 12) and probable recurrent HCV (n = 7) than in definite ACR (n = 7) and other complications (n = 8). There were no significant differences in serum HCV-RNA levels among the four post-transplant conditions. There was no significant correlation between the IHS grades using IG222 mAb and serum HCV-RNA levels when data of 84 liver biopsy specimens were analyzed. Conclusions: Constant HCV-E2 expression was observed in liver biopsy specimens obtained 1 month or longer. The strong HCV-E2 expression on liver grafts were associated with recurrent hepatitis C after LDLT, but the serum HCV-RNA levels were not.",
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AU - Yagi, Takahito

AU - Iwagaki, Hiromi

AU - Matsuda, Hiroaki

AU - Shinoura, Susumu

AU - Umeda, Yuzo

AU - Ohara, Nobuya

AU - Yanai, Hiroyuki

AU - Ogino, Tetsuya

AU - Tanaka, Noriaki

PY - 2009

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AB - Aim: We evaluated the expression of hepatitis C virus (HCV) antigen on liver grafts by immunohistochemical staining (IHS) using IG222 monoclonal antibody (mAb) against HCV-envelope 2 (E2). Methods: The study material was 84 liver biopsy specimens obtained from 28 patients who underwent living donor liver transplantation (LDLT) for HCV infection. The biopsy samples were examined histopathologically, and by IHS using IG222 mAb against HCV-E2. Serum HCV-RNA level was measured in all patients. The IHS grades were compared among the three groups classified according to the time elapsed from LDLT (at 1-30, 31-179 and ≥180 days post-LDLT) and among four post-transplant conditions, including acute cellular rejection (ACR). Results: Immunoreactivity to IG222 was detected in 78.6% of the specimens obtained during the first month after LDLT, and there were no significant differences on the IHS grades between the three groups classified according to the time elapsed from LDLT. The IHS grades were significantly stronger in definite recurrent HCV (n = 12) and probable recurrent HCV (n = 7) than in definite ACR (n = 7) and other complications (n = 8). There were no significant differences in serum HCV-RNA levels among the four post-transplant conditions. There was no significant correlation between the IHS grades using IG222 mAb and serum HCV-RNA levels when data of 84 liver biopsy specimens were analyzed. Conclusions: Constant HCV-E2 expression was observed in liver biopsy specimens obtained 1 month or longer. The strong HCV-E2 expression on liver grafts were associated with recurrent hepatitis C after LDLT, but the serum HCV-RNA levels were not.

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KW - Hepatitis C virus

KW - Immunohistochemical staining

KW - Liver transplantation

KW - Recurrent hepatitis C

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