Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas

Nobuyoshi Mizukawa, Katsumi Sugiyama, Masayuki Kamio, Eiki Yamachika, Takaaki Ueno, Joji Fukunaga, Shin Takagi, Toshio Sugahara

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The purpose of this study was the immunohistochemical localization and distribution of HNP-1 in the submandibular glands of patients with oral carcinomas. Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells in the submandibular glands were stained with anti-defensin antibody. Neutrophils and capillary intimal cells were also stained. Defensins (HNP(s)) are peptides that occur in neutrophils and protect against bacteria and tumor cells. Human α-defensin-1 (HNP-1) is such a peptide, possessing both antimicrobial and cytotoxic activities. The presence of HNP-1 in striated duct cells in the submandibular glands of oral cancer patients, suggests a likely role in tumor immunity, for this peptide.

Original languageEnglish
Pages (from-to)1125-1127
Number of pages3
JournalAnticancer Research
Volume20
Issue number2 B
Publication statusPublished - 2000

Fingerprint

Defensins
Submandibular Gland
Staining and Labeling
Carcinoma
Peptides
Neutrophils
Tunica Intima
Streptavidin
Mouth Neoplasms
Biotin
Paraffin
Peroxidase
Anti-Idiotypic Antibodies
Immunity
Neoplasms
Bacteria
human neutrophil peptide 1

Keywords

  • Defensin
  • HNP-1
  • Oral carcinoma
  • Striated duct cells
  • Submandibular gland

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas. / Mizukawa, Nobuyoshi; Sugiyama, Katsumi; Kamio, Masayuki; Yamachika, Eiki; Ueno, Takaaki; Fukunaga, Joji; Takagi, Shin; Sugahara, Toshio.

In: Anticancer Research, Vol. 20, No. 2 B, 2000, p. 1125-1127.

Research output: Contribution to journalArticle

Mizukawa, N, Sugiyama, K, Kamio, M, Yamachika, E, Ueno, T, Fukunaga, J, Takagi, S & Sugahara, T 2000, 'Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas', Anticancer Research, vol. 20, no. 2 B, pp. 1125-1127.
Mizukawa, Nobuyoshi ; Sugiyama, Katsumi ; Kamio, Masayuki ; Yamachika, Eiki ; Ueno, Takaaki ; Fukunaga, Joji ; Takagi, Shin ; Sugahara, Toshio. / Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas. In: Anticancer Research. 2000 ; Vol. 20, No. 2 B. pp. 1125-1127.
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AU - Ueno, Takaaki

AU - Fukunaga, Joji

AU - Takagi, Shin

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