Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas

Nobuyoshi Mizukawa; Katsumi Sugiyama; Masayuki Kamio; Eiki Yamachika; Takaaki Ueno; Joji Fukunaga; Shin Takagi; Toshio Sugahara

Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas

Nobuyoshi Mizukawa, Katsumi Sugiyama, Masayuki Kamio, Eiki Yamachika, Takaaki Ueno, Joji Fukunaga, Shin Takagi, Toshio Sugahara

University Hospital

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

The purpose of this study was the immunohistochemical localization and distribution of HNP-1 in the submandibular glands of patients with oral carcinomas. Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells in the submandibular glands were stained with anti-defensin antibody. Neutrophils and capillary intimal cells were also stained. Defensins (HNP(s)) are peptides that occur in neutrophils and protect against bacteria and tumor cells. Human α-defensin-1 (HNP-1) is such a peptide, possessing both antimicrobial and cytotoxic activities. The presence of HNP-1 in striated duct cells in the submandibular glands of oral cancer patients, suggests a likely role in tumor immunity, for this peptide.

Original language	English
Pages (from-to)	1125-1127
Number of pages	3
Journal	Anticancer research
Volume	20
Issue number	2 B
Publication status	Published - 2000

Keywords

Defensin
HNP-1
Oral carcinoma
Striated duct cells
Submandibular gland

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas",

abstract = "The purpose of this study was the immunohistochemical localization and distribution of HNP-1 in the submandibular glands of patients with oral carcinomas. Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells in the submandibular glands were stained with anti-defensin antibody. Neutrophils and capillary intimal cells were also stained. Defensins (HNP(s)) are peptides that occur in neutrophils and protect against bacteria and tumor cells. Human α-defensin-1 (HNP-1) is such a peptide, possessing both antimicrobial and cytotoxic activities. The presence of HNP-1 in striated duct cells in the submandibular glands of oral cancer patients, suggests a likely role in tumor immunity, for this peptide.",

keywords = "Defensin, HNP-1, Oral carcinoma, Striated duct cells, Submandibular gland",

author = "Nobuyoshi Mizukawa and Katsumi Sugiyama and Masayuki Kamio and Eiki Yamachika and Takaaki Ueno and Joji Fukunaga and Shin Takagi and Toshio Sugahara",

year = "2000",

language = "English",

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pages = "1125--1127",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

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TY - JOUR

T1 - Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas

AU - Mizukawa, Nobuyoshi

AU - Sugiyama, Katsumi

AU - Kamio, Masayuki

AU - Yamachika, Eiki

AU - Ueno, Takaaki

AU - Fukunaga, Joji

AU - Takagi, Shin

AU - Sugahara, Toshio

PY - 2000

Y1 - 2000

N2 - The purpose of this study was the immunohistochemical localization and distribution of HNP-1 in the submandibular glands of patients with oral carcinomas. Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells in the submandibular glands were stained with anti-defensin antibody. Neutrophils and capillary intimal cells were also stained. Defensins (HNP(s)) are peptides that occur in neutrophils and protect against bacteria and tumor cells. Human α-defensin-1 (HNP-1) is such a peptide, possessing both antimicrobial and cytotoxic activities. The presence of HNP-1 in striated duct cells in the submandibular glands of oral cancer patients, suggests a likely role in tumor immunity, for this peptide.

AB - The purpose of this study was the immunohistochemical localization and distribution of HNP-1 in the submandibular glands of patients with oral carcinomas. Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells in the submandibular glands were stained with anti-defensin antibody. Neutrophils and capillary intimal cells were also stained. Defensins (HNP(s)) are peptides that occur in neutrophils and protect against bacteria and tumor cells. Human α-defensin-1 (HNP-1) is such a peptide, possessing both antimicrobial and cytotoxic activities. The presence of HNP-1 in striated duct cells in the submandibular glands of oral cancer patients, suggests a likely role in tumor immunity, for this peptide.

KW - Defensin

KW - HNP-1

KW - Oral carcinoma

KW - Striated duct cells

KW - Submandibular gland

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M3 - Article

C2 - 10810407

AN - SCOPUS:0034056108

VL - 20

SP - 1125

EP - 1127

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 2 B

ER -

Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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