Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas

Nobuyoshi Mizukawa; Katsumi Sugiyama; Masayuki Kamio; Eiki Yamachika; Takaaki Ueno; Joji Fukunaga; Shin Takagi; Toshio Sugahara

Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas

Nobuyoshi Mizukawa, Katsumi Sugiyama, Masayuki Kamio, Eiki Yamachika, Takaaki Ueno, Joji Fukunaga, Shin Takagi, Toshio Sugahara

University Hospital

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

The purpose of this study was the immunohistochemical localization and distribution of HNP-1 in the submandibular glands of patients with oral carcinomas. Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells in the submandibular glands were stained with anti-defensin antibody. Neutrophils and capillary intimal cells were also stained. Defensins (HNP(s)) are peptides that occur in neutrophils and protect against bacteria and tumor cells. Human α-defensin-1 (HNP-1) is such a peptide, possessing both antimicrobial and cytotoxic activities. The presence of HNP-1 in striated duct cells in the submandibular glands of oral cancer patients, suggests a likely role in tumor immunity, for this peptide.

Original language	English
Pages (from-to)	1125-1127
Number of pages	3
Journal	Anticancer research
Volume	20
Issue number	2 B
Publication status	Published - 2000

Keywords

Defensin
HNP-1
Oral carcinoma
Striated duct cells
Submandibular gland

ASJC Scopus subject areas

Oncology
Cancer Research

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Immunohistochemical staining of human α-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas. / Mizukawa, Nobuyoshi; Sugiyama, Katsumi; Kamio, Masayuki; Yamachika, Eiki; Ueno, Takaaki; Fukunaga, Joji; Takagi, Shin; Sugahara, Toshio.

In: Anticancer research, Vol. 20, No. 2 B, 2000, p. 1125-1127.

Research output: Contribution to journal › Article › peer-review

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abstract = "The purpose of this study was the immunohistochemical localization and distribution of HNP-1 in the submandibular glands of patients with oral carcinomas. Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells in the submandibular glands were stained with anti-defensin antibody. Neutrophils and capillary intimal cells were also stained. Defensins (HNP(s)) are peptides that occur in neutrophils and protect against bacteria and tumor cells. Human α-defensin-1 (HNP-1) is such a peptide, possessing both antimicrobial and cytotoxic activities. The presence of HNP-1 in striated duct cells in the submandibular glands of oral cancer patients, suggests a likely role in tumor immunity, for this peptide.",

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AU - Fukunaga, Joji

AU - Takagi, Shin

AU - Sugahara, Toshio

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