Immunohistochemical observation of heat shock proteins expression in mouse periodontal tissues due to orthodontic mechanical stress

Rina Muraoka, Keisuke Nakano, Hirokazu Matsuda, Maki Tomoda, Norimasa Okafuji, Saburo Kurihara, Kazuhiro Yamada, Toshiyuki Kawakami

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Heat shock proteins (HSPs) are induced by not only the heat shock but also the mechanical stress. Orthodontic tooth movement induced mechanical stress in the related periodontal ligament. It is important to examine the immunohistochemical profile change of the Heat shock proteins (HSPs) in the periodontal ligament cells after receiving the mechanical stress for orthodontic treatment. Therefore, we examined the HSPs in the periodontal ligament cells of ddY mice using the Waldo method. In the control group, periodontal ligament was observed as physiological arrangement, and which reacted weakly to HSP27 and HSP70. In the experimental group the extension site of the periodontal ligament cells and the expansion of the blood vessel occurred in the traction side. These tissues were strongly reacted to HSP27 and HSP70. The findings suggeste that the HSPs expression work as the mechanism of maintenance of homeostasis in the periodontal tissues.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalJournal of Hard Tissue Biology
Volume18
Issue number4
DOIs
Publication statusPublished - Mar 23 2010
Externally publishedYes

Fingerprint

Periodontal Ligament
Mechanical Stress
Ligaments
Heat-Shock Proteins
Orthodontics
Observation
Tissue
Proteins
Tooth Movement Techniques
Blood vessels
Traction
Blood Vessels
Shock
Homeostasis
Hot Temperature
Maintenance
Control Groups

Keywords

  • Heat shock protein
  • HSP27
  • HSP70
  • Mechanical stress
  • Orthodontic tooth movement
  • Periodontal ligament cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Immunohistochemical observation of heat shock proteins expression in mouse periodontal tissues due to orthodontic mechanical stress. / Muraoka, Rina; Nakano, Keisuke; Matsuda, Hirokazu; Tomoda, Maki; Okafuji, Norimasa; Kurihara, Saburo; Yamada, Kazuhiro; Kawakami, Toshiyuki.

In: Journal of Hard Tissue Biology, Vol. 18, No. 4, 23.03.2010, p. 193-198.

Research output: Contribution to journalArticle

Muraoka, Rina ; Nakano, Keisuke ; Matsuda, Hirokazu ; Tomoda, Maki ; Okafuji, Norimasa ; Kurihara, Saburo ; Yamada, Kazuhiro ; Kawakami, Toshiyuki. / Immunohistochemical observation of heat shock proteins expression in mouse periodontal tissues due to orthodontic mechanical stress. In: Journal of Hard Tissue Biology. 2010 ; Vol. 18, No. 4. pp. 193-198.
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