Immunohistochemical observation of heat shock proteins expression in mouse periodontal tissues due to orthodontic mechanical stress

Heat shock proteins (HSPs) are induced by not only the heat shock but also the mechanical stress. Orthodontic tooth movement induced mechanical stress in the related periodontal ligament. It is important to examine the immunohistochemical profile change of the Heat shock proteins (HSPs) in the periodontal ligament cells after receiving the mechanical stress for orthodontic treatment. Therefore, we examined the HSPs in the periodontal ligament cells of ddY mice using the Waldo method. In the control group, periodontal ligament was observed as physiological arrangement, and which reacted weakly to HSP27 and HSP70. In the experimental group the extension site of the periodontal ligament cells and the expansion of the blood vessel occurred in the traction side. These tissues were strongly reacted to HSP27 and HSP70. The findings suggeste that the HSPs expression work as the mechanism of maintenance of homeostasis in the periodontal tissues.