Abstract
The sperm acrosome exhibits a low pH. However, the mechanism of acidification in the acrosome remains unclear. Vacuolar-type proton ATPase (V-ATPase) has been shown to play a principle role in generating and maintaining the acidity of organelles such as lysosomes and endosomes. In this study, we examined whether V-ATPase is localized in the acrosome membranes using immunohistochemical techniques. Sections of rat testis were immunostained using antibodies against V-ATPase. Under light microscopic observation, the perinuclear region in spermatids at an early stage of development was heavily immunostained. At the electron microscopic level, gold particles showing the presence of V-ATPase were localized to the acrosome membranes in the developing spermatids. V-ATPase was also localized to the membrane of vesicles locating between the trans-Golgi area and the acrosome. These observations suggest that V-ATPase may play a role in acrosome acidification.
| Original language | English |
|---|---|
| Pages (from-to) | 278-283 |
| Number of pages | 6 |
| Journal | International Journal of Andrology |
| Volume | 23 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2000 |
| Externally published | Yes |
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Keywords
- Acidification
- Acrosome
- Immunohistochemistry
- Spermatid
- V-ATPase
ASJC Scopus subject areas
- Endocrinology
Cite this
Immunohistochemical localization of V-ATPases in rat spermatids. / Kawa, Gen; Yamamoto, Akitsugu; Yoshimori, Tamotsu; Muguruma, Koei; Matsuda, Tadashi; Moriyama, Yoshinori.
In: International Journal of Andrology, Vol. 23, No. 5, 2000, p. 278-283.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunohistochemical localization of V-ATPases in rat spermatids
AU - Kawa, Gen
AU - Yamamoto, Akitsugu
AU - Yoshimori, Tamotsu
AU - Muguruma, Koei
AU - Matsuda, Tadashi
AU - Moriyama, Yoshinori
PY - 2000
Y1 - 2000
N2 - The sperm acrosome exhibits a low pH. However, the mechanism of acidification in the acrosome remains unclear. Vacuolar-type proton ATPase (V-ATPase) has been shown to play a principle role in generating and maintaining the acidity of organelles such as lysosomes and endosomes. In this study, we examined whether V-ATPase is localized in the acrosome membranes using immunohistochemical techniques. Sections of rat testis were immunostained using antibodies against V-ATPase. Under light microscopic observation, the perinuclear region in spermatids at an early stage of development was heavily immunostained. At the electron microscopic level, gold particles showing the presence of V-ATPase were localized to the acrosome membranes in the developing spermatids. V-ATPase was also localized to the membrane of vesicles locating between the trans-Golgi area and the acrosome. These observations suggest that V-ATPase may play a role in acrosome acidification.
AB - The sperm acrosome exhibits a low pH. However, the mechanism of acidification in the acrosome remains unclear. Vacuolar-type proton ATPase (V-ATPase) has been shown to play a principle role in generating and maintaining the acidity of organelles such as lysosomes and endosomes. In this study, we examined whether V-ATPase is localized in the acrosome membranes using immunohistochemical techniques. Sections of rat testis were immunostained using antibodies against V-ATPase. Under light microscopic observation, the perinuclear region in spermatids at an early stage of development was heavily immunostained. At the electron microscopic level, gold particles showing the presence of V-ATPase were localized to the acrosome membranes in the developing spermatids. V-ATPase was also localized to the membrane of vesicles locating between the trans-Golgi area and the acrosome. These observations suggest that V-ATPase may play a role in acrosome acidification.
KW - Acidification
KW - Acrosome
KW - Immunohistochemistry
KW - Spermatid
KW - V-ATPase
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UR - http://www.scopus.com/inward/citedby.url?scp=0033798078&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2605.2000.00244.x
DO - 10.1046/j.1365-2605.2000.00244.x
M3 - Article
VL - 23
SP - 278
EP - 283
JO - International Journal of Andrology
JF - International Journal of Andrology
SN - 0105-6263
IS - 5
ER -