Immunohistochemical localization of V-ATPases in rat spermatids

Gen Kawa, Akitsugu Yamamoto, Tamotsu Yoshimori, Koei Muguruma, Tadashi Matsuda, Yoshinori Moriyama

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The sperm acrosome exhibits a low pH. However, the mechanism of acidification in the acrosome remains unclear. Vacuolar-type proton ATPase (V-ATPase) has been shown to play a principle role in generating and maintaining the acidity of organelles such as lysosomes and endosomes. In this study, we examined whether V-ATPase is localized in the acrosome membranes using immunohistochemical techniques. Sections of rat testis were immunostained using antibodies against V-ATPase. Under light microscopic observation, the perinuclear region in spermatids at an early stage of development was heavily immunostained. At the electron microscopic level, gold particles showing the presence of V-ATPase were localized to the acrosome membranes in the developing spermatids. V-ATPase was also localized to the membrane of vesicles locating between the trans-Golgi area and the acrosome. These observations suggest that V-ATPase may play a role in acrosome acidification.

Original languageEnglish
Pages (from-to)278-283
Number of pages6
JournalInternational Journal of Andrology
Volume23
Issue number5
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Vacuolar Proton-Translocating ATPases
Acrosome
Spermatids
Adenosine Triphosphatases
Protons
Membranes
Endosomes
Lysosomes
Gold
Organelles
Spermatozoa
Testis
Electrons
Light
Antibodies

Keywords

  • Acidification
  • Acrosome
  • Immunohistochemistry
  • Spermatid
  • V-ATPase

ASJC Scopus subject areas

  • Endocrinology

Cite this

Kawa, G., Yamamoto, A., Yoshimori, T., Muguruma, K., Matsuda, T., & Moriyama, Y. (2000). Immunohistochemical localization of V-ATPases in rat spermatids. International Journal of Andrology, 23(5), 278-283. https://doi.org/10.1046/j.1365-2605.2000.00244.x

Immunohistochemical localization of V-ATPases in rat spermatids. / Kawa, Gen; Yamamoto, Akitsugu; Yoshimori, Tamotsu; Muguruma, Koei; Matsuda, Tadashi; Moriyama, Yoshinori.

In: International Journal of Andrology, Vol. 23, No. 5, 2000, p. 278-283.

Research output: Contribution to journalArticle

Kawa, G, Yamamoto, A, Yoshimori, T, Muguruma, K, Matsuda, T & Moriyama, Y 2000, 'Immunohistochemical localization of V-ATPases in rat spermatids', International Journal of Andrology, vol. 23, no. 5, pp. 278-283. https://doi.org/10.1046/j.1365-2605.2000.00244.x
Kawa, Gen ; Yamamoto, Akitsugu ; Yoshimori, Tamotsu ; Muguruma, Koei ; Matsuda, Tadashi ; Moriyama, Yoshinori. / Immunohistochemical localization of V-ATPases in rat spermatids. In: International Journal of Andrology. 2000 ; Vol. 23, No. 5. pp. 278-283.
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