Immunohistochemical localization of V-ATPases in rat spermatids

Gen Kawa, Akitsugu Yamamoto, Tamotsu Yoshimori, Koei Muguruma, Tadashi Matsuda, Yoshinori Moriyama

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


The sperm acrosome exhibits a low pH. However, the mechanism of acidification in the acrosome remains unclear. Vacuolar-type proton ATPase (V-ATPase) has been shown to play a principle role in generating and maintaining the acidity of organelles such as lysosomes and endosomes. In this study, we examined whether V-ATPase is localized in the acrosome membranes using immunohistochemical techniques. Sections of rat testis were immunostained using antibodies against V-ATPase. Under light microscopic observation, the perinuclear region in spermatids at an early stage of development was heavily immunostained. At the electron microscopic level, gold particles showing the presence of V-ATPase were localized to the acrosome membranes in the developing spermatids. V-ATPase was also localized to the membrane of vesicles locating between the trans-Golgi area and the acrosome. These observations suggest that V-ATPase may play a role in acrosome acidification.

Original languageEnglish
Pages (from-to)278-283
Number of pages6
JournalInternational Journal of Andrology
Issue number5
Publication statusPublished - 2000


  • Acidification
  • Acrosome
  • Immunohistochemistry
  • Spermatid
  • V-ATPase

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Urology


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