Immunohistochemical localization of γ and β subunits of epithelial Na+ channel in the rat molar tooth pulp

H. Ichikawa; T. Fukuda; Ryuji Terayama; T. Yamaai; T. Kuboki; Tomosada Sugimoto

doi:10.1016/j.brainres.2005.10.015

Immunohistochemical localization of γ and β subunits of epithelial Na⁺ channel in the rat molar tooth pulp

H. Ichikawa, T. Fukuda, Ryuji Terayama, T. Yamaai, T. Kuboki, Tomosada Sugimoto

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The distribution of γ and β subunits of epithelial Na ⁺ channel (ENaC), markers for low-threshold mechanoreceptors in peripheral tissues, was examined in the tooth pulp. In the root pulp, γENaC- and βENaC-immunoreactive (IR) nerve fibers showed a thick smooth appearance. These nerve fibers ascended toward the pulp horn and formed subodontoblastic nerve plexuses. Immunoelectron microscopic method revealed that 63% of axons were immunoreactive for γENaC in the root pulp. Virtually all myelinated axons showed γENaC-IR (97%), whereas unmyelinated axons were mostly devoid of it (12%). These findings suggest that myelinated tooth pulp nociceptors respond to mechanical stimuli.

Original language	English
Pages (from-to)	138-141
Number of pages	4
Journal	Brain Research
Volume	1065
Issue number	1-2
DOIs	https://doi.org/10.1016/j.brainres.2005.10.015
Publication status	Published - Dec 14 2005

Keywords

ENaC
Immunohistochemistry
Tooth pulp
Trigeminal ganglion
VRL-1

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/j.brainres.2005.10.015

Cite this

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title = "Immunohistochemical localization of γ and β subunits of epithelial Na+ channel in the rat molar tooth pulp",

abstract = "The distribution of γ and β subunits of epithelial Na + channel (ENaC), markers for low-threshold mechanoreceptors in peripheral tissues, was examined in the tooth pulp. In the root pulp, γENaC- and βENaC-immunoreactive (IR) nerve fibers showed a thick smooth appearance. These nerve fibers ascended toward the pulp horn and formed subodontoblastic nerve plexuses. Immunoelectron microscopic method revealed that 63% of axons were immunoreactive for γENaC in the root pulp. Virtually all myelinated axons showed γENaC-IR (97%), whereas unmyelinated axons were mostly devoid of it (12%). These findings suggest that myelinated tooth pulp nociceptors respond to mechanical stimuli.",

keywords = "ENaC, Immunohistochemistry, Tooth pulp, Trigeminal ganglion, VRL-1",

author = "H. Ichikawa and T. Fukuda and Ryuji Terayama and T. Yamaai and T. Kuboki and Tomosada Sugimoto",

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T1 - Immunohistochemical localization of γ and β subunits of epithelial Na+ channel in the rat molar tooth pulp

AU - Ichikawa, H.

AU - Fukuda, T.

AU - Terayama, Ryuji

AU - Yamaai, T.

AU - Kuboki, T.

AU - Sugimoto, Tomosada

PY - 2005/12/14

Y1 - 2005/12/14

N2 - The distribution of γ and β subunits of epithelial Na + channel (ENaC), markers for low-threshold mechanoreceptors in peripheral tissues, was examined in the tooth pulp. In the root pulp, γENaC- and βENaC-immunoreactive (IR) nerve fibers showed a thick smooth appearance. These nerve fibers ascended toward the pulp horn and formed subodontoblastic nerve plexuses. Immunoelectron microscopic method revealed that 63% of axons were immunoreactive for γENaC in the root pulp. Virtually all myelinated axons showed γENaC-IR (97%), whereas unmyelinated axons were mostly devoid of it (12%). These findings suggest that myelinated tooth pulp nociceptors respond to mechanical stimuli.

AB - The distribution of γ and β subunits of epithelial Na + channel (ENaC), markers for low-threshold mechanoreceptors in peripheral tissues, was examined in the tooth pulp. In the root pulp, γENaC- and βENaC-immunoreactive (IR) nerve fibers showed a thick smooth appearance. These nerve fibers ascended toward the pulp horn and formed subodontoblastic nerve plexuses. Immunoelectron microscopic method revealed that 63% of axons were immunoreactive for γENaC in the root pulp. Virtually all myelinated axons showed γENaC-IR (97%), whereas unmyelinated axons were mostly devoid of it (12%). These findings suggest that myelinated tooth pulp nociceptors respond to mechanical stimuli.

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KW - VRL-1

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