Immunohistochemical expression of osterix appearing in the mouse orthodontic periodontal tension sides

Toshihisa Harada, Keisuke Nakano, Hirokazu Matsuda, Rina Muraoka, Maki Tomoda, Yukiko Yokoi, Kazuhiro Yamada, Norimasa Okafuji

Research output: Contribution to journalArticle

Abstract

Early changes of Osterix expression were examined by immunohistochemistry (IHC) and fluorescent immunohistochemistry (FIHC) in mouse periodontal ligament exposed to experimentally induced orthodontic mechanical stress. Eight-week-old ddY mice were used as experimental animals. To provide continuous orthodontic mechanical stress on the periodontal ligament, a rubber dam sheet was placed between the upper molars of the mice. At 20 min, 1 h, 3 h, 9 h and 24 h after the insertion, related parts of the animal tissues were evaluated by IHC and FIHC regarding Osterix and Runx2 expressions. In the 3-h and 9-h experimental groups, orthodontic tension sites reacted positively. The positive reactions became weak in the experimental group at 24-h. The results suggest that Osterix acts as one of the key factors of osteogenic cell differentiation, which expresses the following after the expression of Runx2, down-steam of Runx2.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalJournal of Hard Tissue Biology
Volume21
Issue number3
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Orthodontics
Immunohistochemistry
Periodontal Ligament
Mechanical Stress
Ligaments
Animals
Rubber Dams
Rubber
Steam
Dams
Cell Differentiation
Tissue

Keywords

  • Immunohistochemistry
  • Orthodontic mechanical stress
  • Osterix
  • Periodontal tissues
  • Runx2

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Immunohistochemical expression of osterix appearing in the mouse orthodontic periodontal tension sides. / Harada, Toshihisa; Nakano, Keisuke; Matsuda, Hirokazu; Muraoka, Rina; Tomoda, Maki; Yokoi, Yukiko; Yamada, Kazuhiro; Okafuji, Norimasa.

In: Journal of Hard Tissue Biology, Vol. 21, No. 3, 2012, p. 321-328.

Research output: Contribution to journalArticle

Harada, T, Nakano, K, Matsuda, H, Muraoka, R, Tomoda, M, Yokoi, Y, Yamada, K & Okafuji, N 2012, 'Immunohistochemical expression of osterix appearing in the mouse orthodontic periodontal tension sides', Journal of Hard Tissue Biology, vol. 21, no. 3, pp. 321-328. https://doi.org/10.2485/jhtb.21.321
Harada, Toshihisa ; Nakano, Keisuke ; Matsuda, Hirokazu ; Muraoka, Rina ; Tomoda, Maki ; Yokoi, Yukiko ; Yamada, Kazuhiro ; Okafuji, Norimasa. / Immunohistochemical expression of osterix appearing in the mouse orthodontic periodontal tension sides. In: Journal of Hard Tissue Biology. 2012 ; Vol. 21, No. 3. pp. 321-328.
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