TY - JOUR
T1 - Immunohistochemical expression of heat shock proteins in the mouse periodontal tissues due to orthodontic mechanical stress
AU - Muraoka, Rina
AU - Nakano, K.
AU - Kurihara, S.
AU - Yamada, K.
AU - Kawakami, T.
N1 - Funding Information:
Acknowledgment: This research was partially supported in part by a Grant-in-aid for Scientific Research (c) (#20592419) from the Japan Society for the Promotion of Science.
PY - 2010/11/25
Y1 - 2010/11/25
N2 - The histopathology of periodontal ligament of the mouse subjected to mechanical stress was studied. Immunohistochemical expressions of HSP27 and p-HSP27 were examined. Experimental animals using the maxillary molars of ddY mouse by Waldo method were used in the study. A separator was inserted to induce mechanical stress. After 10 minutes, 20 minutes, 1 hour, 3 hours, 9 hours and 24 hours, the regional tissues were extracted, fixed in 4% paraformaldehyde and 0.05M phosphate-buffered fixative solution. Paraffin sections were made for immunohistochemistry using HSP27 and p-HSP27. In the control group, the periodontal ligament fibroblasts expressed low HSP27 and p-HSP27. However, in the experimental group, periodontal ligament fibroblasts expressed HSP27 10 minutes after mechanical load application in the tension side. The strongest expression was detected 9 hours after inducing mechanical load. p-HSP27 was also expressed in a time-dependent manner though weaker than HSP27. The findings suggest that HSP27 and p-HSP27 were expressed for the maintenance of homeostasis of periodontal ligament by the activation of periodontal ligament fibroblasts on the tension side. It also suggests that these proteins act as molecular chaperones for osteoblast activation and maintenance of homeostasis.
AB - The histopathology of periodontal ligament of the mouse subjected to mechanical stress was studied. Immunohistochemical expressions of HSP27 and p-HSP27 were examined. Experimental animals using the maxillary molars of ddY mouse by Waldo method were used in the study. A separator was inserted to induce mechanical stress. After 10 minutes, 20 minutes, 1 hour, 3 hours, 9 hours and 24 hours, the regional tissues were extracted, fixed in 4% paraformaldehyde and 0.05M phosphate-buffered fixative solution. Paraffin sections were made for immunohistochemistry using HSP27 and p-HSP27. In the control group, the periodontal ligament fibroblasts expressed low HSP27 and p-HSP27. However, in the experimental group, periodontal ligament fibroblasts expressed HSP27 10 minutes after mechanical load application in the tension side. The strongest expression was detected 9 hours after inducing mechanical load. p-HSP27 was also expressed in a time-dependent manner though weaker than HSP27. The findings suggest that HSP27 and p-HSP27 were expressed for the maintenance of homeostasis of periodontal ligament by the activation of periodontal ligament fibroblasts on the tension side. It also suggests that these proteins act as molecular chaperones for osteoblast activation and maintenance of homeostasis.
KW - HSP27
KW - Heat shock protein
KW - Mechanical stress
KW - Orthodontic tooth movement
KW - Periodontal ligament cells
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U2 - 10.1186/2047-783x-15-11-475
DO - 10.1186/2047-783x-15-11-475
M3 - Article
C2 - 21159572
AN - SCOPUS:78650219457
VL - 15
SP - 475
EP - 482
JO - European Journal of Medical Research
JF - European Journal of Medical Research
SN - 0949-2321
IS - 11
ER -