Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation

S. Saito, K. Nakano, A. Nabeyama, M. Sato, N. Okafuji, A. Yamamoto, E. Kasahara, Toshiyuki Kawakami

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aim: After immediate teeth separation, expression of HSP27 in the mouse dental pulp was examined. Immunohistochemistry was performed to examine the incidence of HSP27 expression. Materials and methods: A total of 36 8-week-old ddY mice were used as experimental subjects and a wedge was inserted in between maxillary right molars. The wedge was removed 30 min or 3 h after insertion. Animals were immediately sacrificed after the removal of wedge or until 1 week later and serial sections from paraffin-embedded tissues were prepared. Immunohistochemistry was carried out to examine the expression of HSP27. The untreated side served as the control. Results: In the control group, the endothelial cells and some pulp fibroblasts weakly expressed HSP27 suggesting that the expression is due to mechanical stress brought about by physiological masticatory force and pressure from the tongue. In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter. The expression decreased at the same level as that of the control group 1 week after wedge removal. Conclusion: HSP27 may serve as an indicator of stimulus strong enough to show its expression.

Original languageEnglish
Pages (from-to)495-500
Number of pages6
JournalEuropean journal of medical research
Volume16
Issue number11
DOIs
Publication statusPublished - Nov 10 2011

Keywords

  • Dental pulp
  • Heat shock protein27
  • Immediate teeth separation
  • Mechanical stress

ASJC Scopus subject areas

  • Medicine(all)

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