Immunohistochemical expression of hard tissue related factors in the mouse dental pulp after immediate teeth separation

A. Nabeyama, Keisuke Nakano, S. Saito, M. Sato, N. Okafuji, A. Yamamoto, E. Kasahara, Toshiyuki Kawakami

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We examined change of Runx2 and ALP expression in mouse tooth pulp which exposed to teeth separation experiment by immunohistochemistry as a model for conservative dentistry treatment. 8-week-old 36 male ddY mice were used and wedge was inserted between upper 1st and 2nd molars. The wedge was removed 30 minutes as well as 3 hours after the insertion and the samples were prepared extending up to 1 week of time period for regular histopathological and immunohistochemical examinations for ALP and Runx2 expression. The opposite sides without wedge insertion were taken as controls. In the control group pulp, weak expressions of Runx2 and ALP in the vessel endothelial cells as well as the pulp cells were revealed, suggesting the appearance of these genes upon mechanical stress induced by mastication and tongue pressure etc. On the other hand in the experiment group, Runx2 expression increased both in 30-minute and 3-hour teeth separation group. The expression became maximum at 24 hours. Then it gradually decreased and became similar level with the control group at 1-week after the wedge insertion. Similarly ALP expression increased after the wedge insertion and was maximum at 24 hours and then gradually decreased to the levels similar with the control group. These results suggest that when immunohistochemical expression of Runx2 as well as ALP was used as an index, no severe damage occur upon clinical application of wedge insertion.

Original languageEnglish
Pages (from-to)507-513
Number of pages7
JournalEuropean Journal of Medical Research
Volume16
Issue number11
Publication statusPublished - Nov 10 2011
Externally publishedYes

Fingerprint

Dental Pulp
Thromboplastin
Tooth
Control Groups
Mechanical Stress
Mastication
Dentistry
Tongue
Endothelial Cells
Immunohistochemistry
Pressure
Genes

Keywords

  • ALP
  • Dental pulp
  • Immediate teeth separation
  • Mechanical stress
  • Runx2

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Immunohistochemical expression of hard tissue related factors in the mouse dental pulp after immediate teeth separation. / Nabeyama, A.; Nakano, Keisuke; Saito, S.; Sato, M.; Okafuji, N.; Yamamoto, A.; Kasahara, E.; Kawakami, Toshiyuki.

In: European Journal of Medical Research, Vol. 16, No. 11, 10.11.2011, p. 507-513.

Research output: Contribution to journalArticle

Nabeyama, A, Nakano, K, Saito, S, Sato, M, Okafuji, N, Yamamoto, A, Kasahara, E & Kawakami, T 2011, 'Immunohistochemical expression of hard tissue related factors in the mouse dental pulp after immediate teeth separation', European Journal of Medical Research, vol. 16, no. 11, pp. 507-513.
Nabeyama, A. ; Nakano, Keisuke ; Saito, S. ; Sato, M. ; Okafuji, N. ; Yamamoto, A. ; Kasahara, E. ; Kawakami, Toshiyuki. / Immunohistochemical expression of hard tissue related factors in the mouse dental pulp after immediate teeth separation. In: European Journal of Medical Research. 2011 ; Vol. 16, No. 11. pp. 507-513.
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