Immunohistochemical evaluation of insulin-like growth factor I receptor status in cervical cancer specimens

Hiroyuki Kuramoto, Atsushi Hongo, Yi Xuan Liu, Yojiro Ojima, Keiichiro Nakamura, Noriko Seki, Junichi Kodama, Yuji Hiramatsu

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20 Citations (Scopus)

Abstract

The insulin-like growth factor I receptor (IGF-IR) is exceptionally overexpressed in many cervical-cancer-derived cell lines. It is postulated that a decrease of p53 protein levels due to human papillomavirus (HPV) infection may contribute to the up-regulation of IGF-IR expression in cervical cancer cells because transcription of IGF-IR is strictly down-regulated by p53. To evaluate this fact in clinical cervical cancer specimens, we checked the expression levels and activated status of IGF-IR by immunohistochemistry. Formalin-fixed and paraffin-embedded specimens obtained by conization or hysterectomy were stained with anti-IGF-IR and with an antibody recognizing phosphorylated tyrosine at its c-terminus. The expression levels of IGF-IR were significantly high in cervical intraepithelial neoplasia (CIN) III and invasive cancer specimens. Phosphorylation of IGF-IR was promoted in all CIN and invasive cancer specimens, and its intensity was related to the promotion of lesions. Interestingly, IGF-IR overexpression was missing in the basal layer of CIN I and II lesions, whereas it was evenly distributed in CIN III and invasive cancer lesions. This IGF-IR overexpression pattern may be utilized in the diagnosis of HPV infection status in CIN lesions. Copyright

Original languageEnglish
Article number5
JournalActa Medica Okayama
Volume62
Issue number4
Publication statusPublished - Aug 2008

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Keywords

  • Cervical cancer
  • Human papillomavirus
  • Insulin-like growth factor I receptor
  • Tyrosil phosphorylation

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kuramoto, H., Hongo, A., Liu, Y. X., Ojima, Y., Nakamura, K., Seki, N., Kodama, J., & Hiramatsu, Y. (2008). Immunohistochemical evaluation of insulin-like growth factor I receptor status in cervical cancer specimens. Acta Medica Okayama, 62(4), [5].