Immunohistochemical changes of heat shock protein 27 expression in the mouse periodontal tissues exposed to orthodontic mechanical stress

Maki Tomoda, Keisuke Nakano, Rina Muraoka, Hirokazu Matsuda, Kazuhiro Yamada, Toshiyuki Kawakami

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Orthodontic mechanical stress was exposed on mouse molars and histopathological changes as well as the expressions of HSP27 and p-HSP27 in the periodontal tissues were examined after removal of mechanical stress. The increased in mechanical stress up to 3 hours led to pathological changes that caused a space in between stretched periodontal ligament fibrous bundles and fibroblasts as well as narrowing of the periodontal ligament space. Degenerative changes were also occurred in the pressure side. Pathological changes did not only occur due to mechanical stress but also at the time of the release of mechanical stress exposure which increased over time. In the control group, both HSP27 and p-HSP27 were negative in the pressure side after mechanical stress was released 3 hours later. On the other hand, the tension side showed a strong positive reaction. The proteins were also expressed after 20 min, 1 hour, 3 hours and 9 hours. The strongest expression was observed 24 hours. A decreased in the intensity of expression was observed 3 days and 1 week later. The results suggest that HSP27 plays an important role in the recovery of injured cells in the periodontal tissues.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalJournal of Hard Tissue Biology
Volume21
Issue number1
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

HSP27 Heat-Shock Proteins
Mechanical Stress
Orthodontics
Tissue
Proteins
Periodontal Ligament
Ligaments
Pressure
Fibroblasts
Hot Temperature
Recovery
Control Groups

Keywords

  • Heat shock protein
  • HSP27
  • Mechanical stress
  • Orthodontic tooth movement
  • Periodontal ligament cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Immunohistochemical changes of heat shock protein 27 expression in the mouse periodontal tissues exposed to orthodontic mechanical stress. / Tomoda, Maki; Nakano, Keisuke; Muraoka, Rina; Matsuda, Hirokazu; Yamada, Kazuhiro; Kawakami, Toshiyuki.

In: Journal of Hard Tissue Biology, Vol. 21, No. 1, 2012, p. 43-50.

Research output: Contribution to journalArticle

Tomoda, Maki ; Nakano, Keisuke ; Muraoka, Rina ; Matsuda, Hirokazu ; Yamada, Kazuhiro ; Kawakami, Toshiyuki. / Immunohistochemical changes of heat shock protein 27 expression in the mouse periodontal tissues exposed to orthodontic mechanical stress. In: Journal of Hard Tissue Biology. 2012 ; Vol. 21, No. 1. pp. 43-50.
@article{72408ba8a6d148db96361af2f77d156b,
title = "Immunohistochemical changes of heat shock protein 27 expression in the mouse periodontal tissues exposed to orthodontic mechanical stress",
abstract = "Orthodontic mechanical stress was exposed on mouse molars and histopathological changes as well as the expressions of HSP27 and p-HSP27 in the periodontal tissues were examined after removal of mechanical stress. The increased in mechanical stress up to 3 hours led to pathological changes that caused a space in between stretched periodontal ligament fibrous bundles and fibroblasts as well as narrowing of the periodontal ligament space. Degenerative changes were also occurred in the pressure side. Pathological changes did not only occur due to mechanical stress but also at the time of the release of mechanical stress exposure which increased over time. In the control group, both HSP27 and p-HSP27 were negative in the pressure side after mechanical stress was released 3 hours later. On the other hand, the tension side showed a strong positive reaction. The proteins were also expressed after 20 min, 1 hour, 3 hours and 9 hours. The strongest expression was observed 24 hours. A decreased in the intensity of expression was observed 3 days and 1 week later. The results suggest that HSP27 plays an important role in the recovery of injured cells in the periodontal tissues.",
keywords = "Heat shock protein, HSP27, Mechanical stress, Orthodontic tooth movement, Periodontal ligament cells",
author = "Maki Tomoda and Keisuke Nakano and Rina Muraoka and Hirokazu Matsuda and Kazuhiro Yamada and Toshiyuki Kawakami",
year = "2012",
doi = "10.2485/jhtb.21.43",
language = "English",
volume = "21",
pages = "43--50",
journal = "Journal of Hard Tissue Biology",
issn = "1341-7649",
publisher = "Society of Hard Tissue Regenerative Biology",
number = "1",

}

TY - JOUR

T1 - Immunohistochemical changes of heat shock protein 27 expression in the mouse periodontal tissues exposed to orthodontic mechanical stress

AU - Tomoda, Maki

AU - Nakano, Keisuke

AU - Muraoka, Rina

AU - Matsuda, Hirokazu

AU - Yamada, Kazuhiro

AU - Kawakami, Toshiyuki

PY - 2012

Y1 - 2012

N2 - Orthodontic mechanical stress was exposed on mouse molars and histopathological changes as well as the expressions of HSP27 and p-HSP27 in the periodontal tissues were examined after removal of mechanical stress. The increased in mechanical stress up to 3 hours led to pathological changes that caused a space in between stretched periodontal ligament fibrous bundles and fibroblasts as well as narrowing of the periodontal ligament space. Degenerative changes were also occurred in the pressure side. Pathological changes did not only occur due to mechanical stress but also at the time of the release of mechanical stress exposure which increased over time. In the control group, both HSP27 and p-HSP27 were negative in the pressure side after mechanical stress was released 3 hours later. On the other hand, the tension side showed a strong positive reaction. The proteins were also expressed after 20 min, 1 hour, 3 hours and 9 hours. The strongest expression was observed 24 hours. A decreased in the intensity of expression was observed 3 days and 1 week later. The results suggest that HSP27 plays an important role in the recovery of injured cells in the periodontal tissues.

AB - Orthodontic mechanical stress was exposed on mouse molars and histopathological changes as well as the expressions of HSP27 and p-HSP27 in the periodontal tissues were examined after removal of mechanical stress. The increased in mechanical stress up to 3 hours led to pathological changes that caused a space in between stretched periodontal ligament fibrous bundles and fibroblasts as well as narrowing of the periodontal ligament space. Degenerative changes were also occurred in the pressure side. Pathological changes did not only occur due to mechanical stress but also at the time of the release of mechanical stress exposure which increased over time. In the control group, both HSP27 and p-HSP27 were negative in the pressure side after mechanical stress was released 3 hours later. On the other hand, the tension side showed a strong positive reaction. The proteins were also expressed after 20 min, 1 hour, 3 hours and 9 hours. The strongest expression was observed 24 hours. A decreased in the intensity of expression was observed 3 days and 1 week later. The results suggest that HSP27 plays an important role in the recovery of injured cells in the periodontal tissues.

KW - Heat shock protein

KW - HSP27

KW - Mechanical stress

KW - Orthodontic tooth movement

KW - Periodontal ligament cells

UR - http://www.scopus.com/inward/record.url?scp=84858592542&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84858592542&partnerID=8YFLogxK

U2 - 10.2485/jhtb.21.43

DO - 10.2485/jhtb.21.43

M3 - Article

AN - SCOPUS:84858592542

VL - 21

SP - 43

EP - 50

JO - Journal of Hard Tissue Biology

JF - Journal of Hard Tissue Biology

SN - 1341-7649

IS - 1

ER -