Immunohistochemical and immunocytochemical localization of amylase in rat parotid glands and von Ebner's glands by ion etching-immunoscanning electron microscopy

Junko Yahiro, Tetsuichiro Inai, Akihito Tsutsui, Atsuko Sato, Toshikazu Nagato, Kunihisa Taniguchi, Eichi Tsuruga, Yoshihiko Sawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The distribution of amylase in rat parotid glands and von Ebner's glands was examined using ion etching-immunoscanning electron microscopy, which enables both light and electron microscopic observations of identical semi-thin resin sections immunolabeled with anti-α- amylase and immunogold in association with silver enhancement. At the light microscopic level, most acinar secretory granules (SG) and striated duct secretions of parotid glands were strongly stained dark brown. In von Ebner's glands, acinar SG and duct secretions were weakly to strongly stained light to dark brown. At the electron microscopic level, labeling was observed as bright gold-silver particles. The labeling intensity of acinar SG of parotid glands was higher than that of von Ebner's glands. In parotid glands, weak labeling of SG in transitional cells between acini and intercalated ducts, very weak labeling of SG in intercalated ducts, and strong labeling of striated duct secretions were observed. In von Ebner's glands, the secretions and some SG of interlobular ducts were strongly labeled compared to those of intralobular ducts and SG of acini. Less amylase was synthesized in von Ebner's acini compared to parotid acini, whereas von Ebner's ducts may secrete significantly more amylase to modify saliva than parotid ducts.

Original languageEnglish
Pages (from-to)201-212
Number of pages12
JournalACTA HISTOCHEMICA ET CYTOCHEMICA
Volume44
Issue number5
DOIs
Publication statusPublished - Aug 10 2011
Externally publishedYes

Keywords

  • Amylase
  • Localization
  • Parotid gland
  • Scanning electron microscopy
  • Von Ebner's gland

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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