Immunohistochemical analysis of the effects of estrogen on intraarticular neurogenic inflammation in a rat anterior cruciate ligament transection model of osteoarthritis

Atsuhiko Yoshida, Toru Morihara, Ken Ichi Matsuda, Hirotaka Sakamoto, Yuji Arai, Yoshikazu Kida, Mitsuhiro Kawata, Toshikazu Kubo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Synovitis is considered as one of the factors associated with the pathogenesis of osteoarthritis (OA). There is currently a significant amount of research linking estrogen deficiencies with the development of OA in estrogen-deficient women, including postmenopausal women; however, the exact etiology remains unclear. Various neuropeptides, such as substance P (SP) and calcitonin gene-related peptide (CGRP), have been shown to contribute to synovitis in OA joints, and the influence of estrogen on the expressions of SP and CGRP in the synovium of OA joints has been noted. After ovariectomy (OVX) followed by estradiol (E2) replacement, 24 female rats were divided into three groups: OVX group, OVX + E2 replacement group (E2 group), and a sham group. All rats underwent transection of the anterior cruciate ligament at the same time. After 30 days, the histological findings of knee joints by hematoxylineosin staining and immunofluorescence staining of protein gene product 9.5 (pan-neuronal marker), SP, and CGRP were compared among experimental groups. The degree of synovitis in the OVX group was higher than in the E2 and sham groups. No significant differences in the density of protein gene product 9.5-immunoreactive nerve fibers were observed among the three experimental groups, but the density of SP- or CGRP-immunoreactive nerve fibers in the OVX group was significantly higher than in the E2 and sham groups. These findings suggest that estrogen partly regulates intraarticular neurogenic inflammation in OA joints by modulating the expressions of neuropeptides in the synovium.

Original languageEnglish
Pages (from-to)197-206
Number of pages10
JournalConnective Tissue Research
Volume53
Issue number3
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Neurogenic Inflammation
Calcitonin Gene-Related Peptide
Anterior Cruciate Ligament
Ligaments
Substance P
Osteoarthritis
Rats
Estrogens
Synovitis
Neuropeptides
Synovial Membrane
Joints
Nerve Fibers
Fibers
Staining and Labeling
Ovariectomy
Estradiol
Knee Joint
Proteins
Fluorescent Antibody Technique

Keywords

  • Estrogen
  • Immunohistochemistry
  • Neuropeptide
  • Osteoarthritis
  • Synovium

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Immunohistochemical analysis of the effects of estrogen on intraarticular neurogenic inflammation in a rat anterior cruciate ligament transection model of osteoarthritis. / Yoshida, Atsuhiko; Morihara, Toru; Matsuda, Ken Ichi; Sakamoto, Hirotaka; Arai, Yuji; Kida, Yoshikazu; Kawata, Mitsuhiro; Kubo, Toshikazu.

In: Connective Tissue Research, Vol. 53, No. 3, 06.2012, p. 197-206.

Research output: Contribution to journalArticle

Yoshida, Atsuhiko ; Morihara, Toru ; Matsuda, Ken Ichi ; Sakamoto, Hirotaka ; Arai, Yuji ; Kida, Yoshikazu ; Kawata, Mitsuhiro ; Kubo, Toshikazu. / Immunohistochemical analysis of the effects of estrogen on intraarticular neurogenic inflammation in a rat anterior cruciate ligament transection model of osteoarthritis. In: Connective Tissue Research. 2012 ; Vol. 53, No. 3. pp. 197-206.
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