Abstract
Immunohistochemistry is a major technique to determine the distribution and localization of differentially produced proteins in the context of an intact tissue. It exploits one of the properties of antibodies, specific binding to an antigen, i.e., to the epitope of its target protein, in combination with a color-developing enzymatic reaction or tagged fluorophore. We have clarified the spatial and temporal expression patterns of CCN family proteins in several different types of animal tissues by using this immunohistochemical technique to support our corresponding data obtained in vitro. In this chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 53-62 |
| Number of pages | 10 |
| Volume | 1489 |
| DOIs | |
| Publication status | Published - 2017 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1489 |
| ISSN (Print) | 10643745 |
Fingerprint
Keywords
- Antigen retrieving
- Masked epitope
- Monitoring protein distribution
- Paraffin-embedded sections
- Tissue morphology
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Immunohistochemical analysis of CCN proteins in calcified tissues. / Kawaki, Harumi; Kubota, Satoshi; Takigawa, Masaharu.
Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 53-62 (Methods in Molecular Biology; Vol. 1489).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Immunohistochemical analysis of CCN proteins in calcified tissues
AU - Kawaki, Harumi
AU - Kubota, Satoshi
AU - Takigawa, Masaharu
PY - 2017
Y1 - 2017
N2 - Immunohistochemistry is a major technique to determine the distribution and localization of differentially produced proteins in the context of an intact tissue. It exploits one of the properties of antibodies, specific binding to an antigen, i.e., to the epitope of its target protein, in combination with a color-developing enzymatic reaction or tagged fluorophore. We have clarified the spatial and temporal expression patterns of CCN family proteins in several different types of animal tissues by using this immunohistochemical technique to support our corresponding data obtained in vitro. In this chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family.
AB - Immunohistochemistry is a major technique to determine the distribution and localization of differentially produced proteins in the context of an intact tissue. It exploits one of the properties of antibodies, specific binding to an antigen, i.e., to the epitope of its target protein, in combination with a color-developing enzymatic reaction or tagged fluorophore. We have clarified the spatial and temporal expression patterns of CCN family proteins in several different types of animal tissues by using this immunohistochemical technique to support our corresponding data obtained in vitro. In this chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family.
KW - Antigen retrieving
KW - Masked epitope
KW - Monitoring protein distribution
KW - Paraffin-embedded sections
KW - Tissue morphology
UR - http://www.scopus.com/inward/record.url?scp=84991583703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991583703&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6430-7_6
DO - 10.1007/978-1-4939-6430-7_6
M3 - Chapter
C2 - 27734365
AN - SCOPUS:84991583703
VL - 1489
T3 - Methods in Molecular Biology
SP - 53
EP - 62
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -