Immunohistochemical analysis of CCN proteins in calcified tissues

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Immunohistochemistry is a major technique to determine the distribution and localization of differentially produced proteins in the context of an intact tissue. It exploits one of the properties of antibodies, specific binding to an antigen, i.e., to the epitope of its target protein, in combination with a color-developing enzymatic reaction or tagged fluorophore. We have clarified the spatial and temporal expression patterns of CCN family proteins in several different types of animal tissues by using this immunohistochemical technique to support our corresponding data obtained in vitro. In this chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages53-62
Number of pages10
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

Fingerprint

CCN Intercellular Signaling Proteins
Immunohistochemistry
Antibodies
Paraffin
Epitopes
Proteins
Color
Antigens
In Vitro Techniques

Keywords

  • Antigen retrieving
  • Masked epitope
  • Monitoring protein distribution
  • Paraffin-embedded sections
  • Tissue morphology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kawaki, H., Kubota, S., & Takigawa, M. (2017). Immunohistochemical analysis of CCN proteins in calcified tissues. In Methods in Molecular Biology (Vol. 1489, pp. 53-62). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_6

Immunohistochemical analysis of CCN proteins in calcified tissues. / Kawaki, Harumi; Kubota, Satoshi; Takigawa, Masaharu.

Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. p. 53-62 (Methods in Molecular Biology; Vol. 1489).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kawaki, H, Kubota, S & Takigawa, M 2017, Immunohistochemical analysis of CCN proteins in calcified tissues. in Methods in Molecular Biology. vol. 1489, Methods in Molecular Biology, vol. 1489, Humana Press Inc., pp. 53-62. https://doi.org/10.1007/978-1-4939-6430-7_6
Kawaki H, Kubota S, Takigawa M. Immunohistochemical analysis of CCN proteins in calcified tissues. In Methods in Molecular Biology. Vol. 1489. Humana Press Inc. 2017. p. 53-62. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6430-7_6
Kawaki, Harumi ; Kubota, Satoshi ; Takigawa, Masaharu. / Immunohistochemical analysis of CCN proteins in calcified tissues. Methods in Molecular Biology. Vol. 1489 Humana Press Inc., 2017. pp. 53-62 (Methods in Molecular Biology).
@inbook{50fa8033429f45f8b10fb1ed0052fded,
title = "Immunohistochemical analysis of CCN proteins in calcified tissues",
abstract = "Immunohistochemistry is a major technique to determine the distribution and localization of differentially produced proteins in the context of an intact tissue. It exploits one of the properties of antibodies, specific binding to an antigen, i.e., to the epitope of its target protein, in combination with a color-developing enzymatic reaction or tagged fluorophore. We have clarified the spatial and temporal expression patterns of CCN family proteins in several different types of animal tissues by using this immunohistochemical technique to support our corresponding data obtained in vitro. In this chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family.",
keywords = "Antigen retrieving, Masked epitope, Monitoring protein distribution, Paraffin-embedded sections, Tissue morphology",
author = "Harumi Kawaki and Satoshi Kubota and Masaharu Takigawa",
year = "2017",
doi = "10.1007/978-1-4939-6430-7_6",
language = "English",
volume = "1489",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "53--62",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Immunohistochemical analysis of CCN proteins in calcified tissues

AU - Kawaki, Harumi

AU - Kubota, Satoshi

AU - Takigawa, Masaharu

PY - 2017

Y1 - 2017

N2 - Immunohistochemistry is a major technique to determine the distribution and localization of differentially produced proteins in the context of an intact tissue. It exploits one of the properties of antibodies, specific binding to an antigen, i.e., to the epitope of its target protein, in combination with a color-developing enzymatic reaction or tagged fluorophore. We have clarified the spatial and temporal expression patterns of CCN family proteins in several different types of animal tissues by using this immunohistochemical technique to support our corresponding data obtained in vitro. In this chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family.

AB - Immunohistochemistry is a major technique to determine the distribution and localization of differentially produced proteins in the context of an intact tissue. It exploits one of the properties of antibodies, specific binding to an antigen, i.e., to the epitope of its target protein, in combination with a color-developing enzymatic reaction or tagged fluorophore. We have clarified the spatial and temporal expression patterns of CCN family proteins in several different types of animal tissues by using this immunohistochemical technique to support our corresponding data obtained in vitro. In this chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family.

KW - Antigen retrieving

KW - Masked epitope

KW - Monitoring protein distribution

KW - Paraffin-embedded sections

KW - Tissue morphology

UR - http://www.scopus.com/inward/record.url?scp=84991583703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991583703&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6430-7_6

DO - 10.1007/978-1-4939-6430-7_6

M3 - Chapter

C2 - 27734365

AN - SCOPUS:84991583703

VL - 1489

T3 - Methods in Molecular Biology

SP - 53

EP - 62

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -