Immunohistochemical analysis of CCN proteins in calcified tissues

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Immunohistochemistry is a major technique to determine the distribution and localization of differentially produced proteins in the context of an intact tissue. It exploits one of the properties of antibodies, specific binding to an antigen, i.e., to the epitope of its target protein, in combination with a color-developing enzymatic reaction or tagged fluorophore. We have clarified the spatial and temporal expression patterns of CCN family proteins in several different types of animal tissues by using this immunohistochemical technique to support our corresponding data obtained in vitro. In this chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages53-62
Number of pages10
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)1064-3745

Keywords

  • Antigen retrieving
  • Masked epitope
  • Monitoring protein distribution
  • Paraffin-embedded sections
  • Tissue morphology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kawaki, H., Kubota, S., & Takigawa, M. (2017). Immunohistochemical analysis of CCN proteins in calcified tissues. In Methods in Molecular Biology (pp. 53-62). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_6