Immunoglobulin G1 antibody response to Helicobacter pylori heat shock protein 60 is closely associated with low-grade gastric mucosa-associated lymphoid tissue lymphoma

E. Ishii, Kenji Yokota, T. Sugiyama, Y. Fujinaga, K. Ayada, I. Hokari, S. Hayashi, Y. Hirai, M. Asaka, K. Oguma

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Abstract

Gastric mucosa-associated lymphoid tissue (MALT) lymphoma is related to Helicobacter pylori infection. Specifically, it has been pointed out that pathogenesis of MALT lymphoma involves the 60-kDa heat shock protein (hsp60). To investigate humoral immune responses to the H. pylori hsp60 in patients with gastroduodenal diseases and patients with MALT lymphoma, the hsp60 of H. pylori was expressed with a glutathione S-transferase fusion protein and was purified (recombinant hsp60). Sera were obtained from H. pylori-positive patients with gastroduodenal diseases (MALT lymphoma, n = 13; gastric ulcer, n = 20; duodenal ulcer, n = 20; gastritis, n = 20) and from H. pylori-negative healthy volunteers (n = 9). Sera from patients with MALT lymphoma were also obtained at two times: before and after eradication therapy. Antibodies to hsp60 and H. pylori were assessed by enzyme-linked immunosorbent assay. The levels of immunoglobulin G (IgG) antibodies to the hsp60 of H. pylori-positive patients with gastroduodenal diseases were significantly elevated compared to those in the controls. The levels of IgG1 antibodies to hsp60 were elevated and correlated with the levels of anti-H. pylori antibodies in patients with MALT lymphoma. Humarol immunity against hsp60 may be important and relevant to gastroduodenal diseases induced by H. pylori infection.

Original languageEnglish
Pages (from-to)1056-1059
Number of pages4
JournalClinical and Diagnostic Laboratory Immunology
Volume8
Issue number6
DOIs
Publication statusPublished - 2001

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Chaperonin 60
Marginal Zone B-Cell Lymphoma
Gastric Mucosa
Helicobacter pylori
Antibody Formation
Immunoglobulins
Tissue
Antibodies
Immunoglobulin G
Helicobacter Infections
Immunosorbents
Heat-Shock Proteins
Glutathione Transferase
Mucous Membrane
Assays
Fusion reactions
Gastritis
Stomach Ulcer
Duodenal Ulcer
Humoral Immunity

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Immunoglobulin G1 antibody response to Helicobacter pylori heat shock protein 60 is closely associated with low-grade gastric mucosa-associated lymphoid tissue lymphoma. / Ishii, E.; Yokota, Kenji; Sugiyama, T.; Fujinaga, Y.; Ayada, K.; Hokari, I.; Hayashi, S.; Hirai, Y.; Asaka, M.; Oguma, K.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 8, No. 6, 2001, p. 1056-1059.

Research output: Contribution to journalArticle

Ishii, E. ; Yokota, Kenji ; Sugiyama, T. ; Fujinaga, Y. ; Ayada, K. ; Hokari, I. ; Hayashi, S. ; Hirai, Y. ; Asaka, M. ; Oguma, K. / Immunoglobulin G1 antibody response to Helicobacter pylori heat shock protein 60 is closely associated with low-grade gastric mucosa-associated lymphoid tissue lymphoma. In: Clinical and Diagnostic Laboratory Immunology. 2001 ; Vol. 8, No. 6. pp. 1056-1059.
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