Immunoglobulin G Fc Fragment‐Binding Proteins in Mycoplasma salivarium Cells

Yoshihiko Sawa, Tsuguo Watanabe, Ken‐ichiro ‐i Shibata

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Cell proteins of Mycoplasma salivarium were separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, transferred to membranes, then examined for reactivity with human IgG molecules, the Fc fragment of human IgG, and concanavalin A (ConA). Multiple protein bands bound IgG, and most of them also bound ConA. One (corresponding to a molecular mass of 90 kDa) of the IgG‐ and ConA‐binding bands intensely interacted with the Fc fragment of IgG. The reactivity of proteins eluted from the band with the Fc fragment, tested by dot‐blotting and ELISA, was inhibited (90%) by pre‐incubation with IgG and to a lesser extent (50%), with IgM. Thus, M. salivarium contained a cellular protein with a molecular mass of 90 kDa, that bound the Fc fragment of human IgG.

Original languageEnglish
Pages (from-to)655-659
Number of pages5
JournalMICROBIOLOGY and IMMUNOLOGY
Volume36
Issue number6
DOIs
Publication statusPublished - Jun 1992
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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