Immunoglobulin G Fc Fragment-Binding Proteins in Mycoplasma salivarium Cells

Yoshihiko Sawa, Tsuguo Watanabe, Ken ichiro Shibata

Research output: Contribution to journalArticle


Cell proteins of Mycoplasma salivarium were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to membranes, then examined for reactivity with human IgG molecules, the Fc fragment of human IgG, and concanavalin A (ConA). Multiple protein bands bound IgG, and most of them also bound ConA. One (corresponding to a molecular mass of 90 kDa) of the IgG- and ConA-binding bands intensely interacted with the Fc fragment of IgG. The reactivity of proteins eluted from the band with the Fc fragment, tested by dot-blotting and ELISA, was inhibited (90%) by pre-incubation with IgG and to a lesser extent (50%), with IgM. Thus, M. salivarium contained a cellular protein with a molecular mass of 90 kDa, that bound the Fc fragment of human IgG.

Original languageEnglish
Pages (from-to)575-581
Number of pages7
JournalMicrobiology and Immunology
Issue number6
Publication statusPublished - 1992
Externally publishedYes


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this