Immunoglobulin expressions are only associated with MCPyV-positive Merkel cell carcinomas but not with MCPyV-negative ones: Comparison of prognosis

Ichiro Murakami, Katsuyoshi Takata, Michiko Matsushita, Daisuke Nonaka, Takeshi Iwasaki, Satoshi Kuwamoto, Masako Kato, Takashi Mohri, Keiko Nagata, Yukisato Kitamura, Tadashi Yoshino, Kazuhiko Hayashi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Merkel cell carcinoma (MCC) is an aggressive neuroendocrine skin cancer, often associated with Merkel cell polyomavirus (MCPyV). Recently, immunoglobulin (Ig) expression was reported in MCC, thereby suggesting that B cells might be their cellular ancestors. We tested 30 MCCs (20 MCPyV-positive and 10 MCPyV-negative) using immunohistochemistry for the expressions of IgG, IgA, IgM, Igκ, Igλ, terminal desoxynucleotidyl transferase, paired box gene 5(PAX5), octamer transcription factor-2 (Oct-2), and sex-determining region Y-box 11 (SOX11). We performed in situ hybridization for IgκmRNA or Igλ-mRNA and Ig heavy chain (IgH) gene rearrangement (IgH-R) analyses. The expressions of PAX5, TdT, Oct-2, and SOX11 were not significantly different between MCPyV-positive and MCPyV-negative MCCs. At least 1 of IgG, IgA, IgM, or Igκ was expressed in MCPyV-positive (14/20, 70%) and none in MCPyV-negative MCCs (P=0.0003). There was a higher tendency for Igk-mRNA expression (7/19, using in situ hybridization) and IgH-R (10/20, using polymerase chain reaction) in MCPyV-positive than in MCPyV-negative MCCs (0/10 and 2/10, respectively), thus suggesting a different Ig production pattern and pathogenesis between the 2 types of MCC. Ig expression or IgH-R in MCPyV-positive MCCs might be associated with MCPyV gene integration or expression in cancer cells but do not necessarily suggest a B-cell origin for MCCs. IgH expression or IgH-R nonsignificantly correlated with improved prognosis. However, these might be important factors that influence the survival of neoplastic cells and might allow the development of novel therapies for patients with MCPyV-positive MCCs.

Original languageEnglish
Pages (from-to)1627-1635
Number of pages9
JournalAmerican Journal of Surgical Pathology
Volume38
Issue number12
Publication statusPublished - 2014

Fingerprint

Merkel cell polyomavirus
Merkel Cell Carcinoma
Immunoglobulins
Immunoglobulin Heavy Chains
Octamer Transcription Factor-2
Immunoglobulin A
In Situ Hybridization
Immunoglobulin M
PAX5 Transcription Factor
B-Lymphocytes
Immunoglobulin G
Immunoglobulin Heavy Chain Genes
Messenger RNA
DNA Nucleotidylexotransferase
Gene Rearrangement
Skin Neoplasms

Keywords

  • Igh rearrangement
  • Immunoglobulin gene expressions
  • Merkel cell carcinoma
  • Merkel cell polyomavirus

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Surgery
  • Medicine(all)

Cite this

Murakami, I., Takata, K., Matsushita, M., Nonaka, D., Iwasaki, T., Kuwamoto, S., ... Hayashi, K. (2014). Immunoglobulin expressions are only associated with MCPyV-positive Merkel cell carcinomas but not with MCPyV-negative ones: Comparison of prognosis. American Journal of Surgical Pathology, 38(12), 1627-1635.

Immunoglobulin expressions are only associated with MCPyV-positive Merkel cell carcinomas but not with MCPyV-negative ones : Comparison of prognosis. / Murakami, Ichiro; Takata, Katsuyoshi; Matsushita, Michiko; Nonaka, Daisuke; Iwasaki, Takeshi; Kuwamoto, Satoshi; Kato, Masako; Mohri, Takashi; Nagata, Keiko; Kitamura, Yukisato; Yoshino, Tadashi; Hayashi, Kazuhiko.

In: American Journal of Surgical Pathology, Vol. 38, No. 12, 2014, p. 1627-1635.

Research output: Contribution to journalArticle

Murakami, I, Takata, K, Matsushita, M, Nonaka, D, Iwasaki, T, Kuwamoto, S, Kato, M, Mohri, T, Nagata, K, Kitamura, Y, Yoshino, T & Hayashi, K 2014, 'Immunoglobulin expressions are only associated with MCPyV-positive Merkel cell carcinomas but not with MCPyV-negative ones: Comparison of prognosis', American Journal of Surgical Pathology, vol. 38, no. 12, pp. 1627-1635.
Murakami, Ichiro ; Takata, Katsuyoshi ; Matsushita, Michiko ; Nonaka, Daisuke ; Iwasaki, Takeshi ; Kuwamoto, Satoshi ; Kato, Masako ; Mohri, Takashi ; Nagata, Keiko ; Kitamura, Yukisato ; Yoshino, Tadashi ; Hayashi, Kazuhiko. / Immunoglobulin expressions are only associated with MCPyV-positive Merkel cell carcinomas but not with MCPyV-negative ones : Comparison of prognosis. In: American Journal of Surgical Pathology. 2014 ; Vol. 38, No. 12. pp. 1627-1635.
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abstract = "Merkel cell carcinoma (MCC) is an aggressive neuroendocrine skin cancer, often associated with Merkel cell polyomavirus (MCPyV). Recently, immunoglobulin (Ig) expression was reported in MCC, thereby suggesting that B cells might be their cellular ancestors. We tested 30 MCCs (20 MCPyV-positive and 10 MCPyV-negative) using immunohistochemistry for the expressions of IgG, IgA, IgM, Igκ, Igλ, terminal desoxynucleotidyl transferase, paired box gene 5(PAX5), octamer transcription factor-2 (Oct-2), and sex-determining region Y-box 11 (SOX11). We performed in situ hybridization for IgκmRNA or Igλ-mRNA and Ig heavy chain (IgH) gene rearrangement (IgH-R) analyses. The expressions of PAX5, TdT, Oct-2, and SOX11 were not significantly different between MCPyV-positive and MCPyV-negative MCCs. At least 1 of IgG, IgA, IgM, or Igκ was expressed in MCPyV-positive (14/20, 70{\%}) and none in MCPyV-negative MCCs (P=0.0003). There was a higher tendency for Igk-mRNA expression (7/19, using in situ hybridization) and IgH-R (10/20, using polymerase chain reaction) in MCPyV-positive than in MCPyV-negative MCCs (0/10 and 2/10, respectively), thus suggesting a different Ig production pattern and pathogenesis between the 2 types of MCC. Ig expression or IgH-R in MCPyV-positive MCCs might be associated with MCPyV gene integration or expression in cancer cells but do not necessarily suggest a B-cell origin for MCCs. IgH expression or IgH-R nonsignificantly correlated with improved prognosis. However, these might be important factors that influence the survival of neoplastic cells and might allow the development of novel therapies for patients with MCPyV-positive MCCs.",
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AU - Nonaka, Daisuke

AU - Iwasaki, Takeshi

AU - Kuwamoto, Satoshi

AU - Kato, Masako

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AU - Nagata, Keiko

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AU - Yoshino, Tadashi

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