Immunoelectron microscopic study of PECAM-1 expression on lymphatic endothelium of the human tongue

N. Ebata, Yoshihiko Sawa, Y. Nodasaka, Y. Yamaoka, S. Yoshida, Y. Totsuka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The expression of platelet-endothelial cell adhesion molecule-1 (PECAM-1) on lymphatic and blood vessels of the human tongue was examined with fluorescence and transmission electron microscopy (TEM). The study used anti-desmoplakins antiserum for light microscopic identification of the lymphatic vessels, plus a pre-embedding immunogold electron microscopic technique for TEM observations. Before making TEM observations, cryostat serial sections were immunostained with anti-desmoplakins or anti-PECAM-1 and then embedded. Semithin sections from each cryostat section were photographed under a light microscope and compared in order to identify the lymphatic vessels expressing PECAM-1. In fluorescence microscopy, PECAM-1 expression on lymphatic vessels was weaker than that on blood vessels. TEM observations showed that PECAM-1 expression on the blood vessels was observed only on the luminal surface of the endothelium. In lymphatic vessels, PECAM-1 expression was found both on the luminal and abluminal surfaces of the endothelium. The density of the PECAM-1 reaction products was lower in lymphatic vessels than in blood vessels. The density of PECAM-1 reaction products on the luminal surface of lymphatic vessels was higher than on the abluminal surfaces. The results suggest that blood vessels are more active than lymphatic vessels in leukocyte migration. The expression of PECAM-1 on the abluminal surface of lymphatic endothelium may allow leukocytes to adhere to the endothelium and interact in their migration from tissue into lymphatic vessels.

Original languageEnglish
Pages (from-to)211-218
Number of pages8
JournalTissue and Cell
Volume33
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Lymphatic Endothelium
CD31 Antigens
Lymphatic Vessels
Tongue
Blood Vessels
Transmission Electron Microscopy
Desmoplakins
Endothelium
Leukocytes
Light
Fluorescence Microscopy
Immune Sera
Fluorescence
Electrons

Keywords

  • Human tongue
  • Immuno-electron microscopy
  • Lymphatic vessel
  • PECAM-1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Immunoelectron microscopic study of PECAM-1 expression on lymphatic endothelium of the human tongue. / Ebata, N.; Sawa, Yoshihiko; Nodasaka, Y.; Yamaoka, Y.; Yoshida, S.; Totsuka, Y.

In: Tissue and Cell, Vol. 33, No. 3, 2001, p. 211-218.

Research output: Contribution to journalArticle

Ebata, N. ; Sawa, Yoshihiko ; Nodasaka, Y. ; Yamaoka, Y. ; Yoshida, S. ; Totsuka, Y. / Immunoelectron microscopic study of PECAM-1 expression on lymphatic endothelium of the human tongue. In: Tissue and Cell. 2001 ; Vol. 33, No. 3. pp. 211-218.
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