Immunochemical properties of glucosyltransferases from Streptococcus mutans

K. Fukui, Susumu Kokeguchi, K. Kato, Y. Miyake, R. Nogami, T. Moriyama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Antserum against purified mutansynthetase (EC 2.4.1.?) of Streptococcus mutans 6715 (serotype g), which is responsible for the synthesis of water-insoluble glucan (ISG) in the presence of both sucrose and water-soluble glucan, was prepared. The specificity of the antiserum was tested by using crude enzyme preparations (CEPs) of S. mutans strains of various serotypes. On immunodiffusion, the antiserum cross-reacted with CEPs from strains of serotypes a (HS-6 and AHT), d (OMZ176), and g (OMZ65 and KIR), but not with those from strains of serotypes b (BHT and FA-1) and c (GS-5 and Ingbritt). The antiserum inhibited the synthesis of ISG by crude or purified mutansynthetase of S. mutans 6715. The activities of ISG synthesis by CEPs from the strains antigenically related in the foregoing immunodiffusion were inhibited by the antiserum against strain 6715 mutansynthetase. The antiserum, however, also inhibited the enzyme activity of the strains of serotype b. The finding that the antiserum against purified dextransucrase of S. mutans HS-6 inhibited ISG synthesis by a CEP of strain HS-6 and also by CEPs of antigenically related strains suggested that dextransucrase activity is involved in ISG synthesis.

Original languageEnglish
Pages (from-to)762-766
Number of pages5
JournalInfection and Immunity
Volume39
Issue number2
Publication statusPublished - 1983

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Glucosyltransferases
HS 6
Streptococcus mutans
Glucans
Immune Sera
Enzymes
Immunodiffusion
Butylated Hydroxytoluene
Water
Sucrose
Serogroup

ASJC Scopus subject areas

  • Immunology

Cite this

Fukui, K., Kokeguchi, S., Kato, K., Miyake, Y., Nogami, R., & Moriyama, T. (1983). Immunochemical properties of glucosyltransferases from Streptococcus mutans. Infection and Immunity, 39(2), 762-766.

Immunochemical properties of glucosyltransferases from Streptococcus mutans. / Fukui, K.; Kokeguchi, Susumu; Kato, K.; Miyake, Y.; Nogami, R.; Moriyama, T.

In: Infection and Immunity, Vol. 39, No. 2, 1983, p. 762-766.

Research output: Contribution to journalArticle

Fukui, K, Kokeguchi, S, Kato, K, Miyake, Y, Nogami, R & Moriyama, T 1983, 'Immunochemical properties of glucosyltransferases from Streptococcus mutans', Infection and Immunity, vol. 39, no. 2, pp. 762-766.
Fukui, K. ; Kokeguchi, Susumu ; Kato, K. ; Miyake, Y. ; Nogami, R. ; Moriyama, T. / Immunochemical properties of glucosyltransferases from Streptococcus mutans. In: Infection and Immunity. 1983 ; Vol. 39, No. 2. pp. 762-766.
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