Immunochemical detection of food-derived isothiocyanate as a lysine conjugate

Toshiyuki Nakamura, Noritoshi Kitamoto, Toshihiko Osawa, Yoji Kato

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In a previous study we prepared monoclonal antibody against allyl isothiocyanate (AITC)-modified lysine (Lys), and found that AITC reacted with Lys under physiological conditions in vitro (T. Nakamura et al., Chem. Res. Toxicol., 22, 536-542 (2009)). In the present study, antibodies against benzyl isothiocyanate (ITC), 6-methylsulfinylhexyl ITC and phenethyl ITC modified protein were prepared, and the respective monoclonal antibodies, B6C9, 6MS3D10, and PE3A10 were obtained. These antibodies were applied to ITC detection in food using shredded Wasabia japonica (wasabi) and ground Carica papaya (papaya) seed by trapping ITC with biotin-labeled bovine serum albumin. ITC formation from the wasabi and papaya seed samples was confirmed using the antibodies in a dose-dependent manner. These antibodies might be applicable in identifying food-derived ITC.

Original languageEnglish
Pages (from-to)536-540
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number3
DOIs
Publication statusPublished - Apr 1 2010
Externally publishedYes

Keywords

  • Antibody
  • Enzyme-linked immunosorbent assay
  • Isothiocyanate
  • Lysine adduct

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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