Immunobiological activities of a porin fraction isolated from Fusobacterium nucleatum ATCC 10953

H. Takada, T. Ogawa, F. Yoshimura, K. Otsuka, Susumu Kokeguchi, K. Kato, T. Umemoto, S. Kotani

Research output: Contribution to journalArticle

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Abstract

From Fusobacterium nucleatum ATCC 10953 cell envelope fraction whose inner membranes had been removed by treatment with sodium N-lauroyl sarcosinate, an outer membrane protein (37,000 M(r) in a native state) was prepared by extraction with lithium dodecyl sulfate. The protein thus obtained showed distinct porin activity, namely, the ability to form hydrophilic diffusion pores by incorporation into the artificial liposome membrane. The porin fraction exhibited strong immunobiological activities in the in vitro assays: B-cell mitogenicity and polyclonal B-cell activation on murine splenocytes, stimulatory effects on guinea pig peritoneal macrophages, and enhancement of the migration of human blood monocytes. The porin fraction also exhibited immunoadjuvant activity to increase the antibody production against sheep erythrocytes in the spleen of mice that were immunized by sheep erythrocytes with porin. Although chemical analyses revealed that the test porin fraction contained a considerable amount of lipopolysaccharide (LPS) (around 12% of the fraction), the studies with LPS-nonresponding C3H/HeJ mice and on the inhibitory effects of polymyxin B strongly suggest that most of the above bioactivities are due to porin protein itself, not to coexistent LPS in the porin fraction.

Original languageEnglish
Pages (from-to)855-863
Number of pages9
JournalInfection and Immunity
Volume56
Issue number4
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Fusobacterium nucleatum
Porins
Lipopolysaccharides
Sheep
B-Lymphocytes
Erythrocytes
Artificial Membranes
Immunologic Adjuvants
Polymyxin B
Inbred C3H Mouse
Peritoneal Macrophages
Liposomes
Antibody Formation
Monocytes
Guinea Pigs
Membrane Proteins
Proteins
Spleen
Sodium
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Takada, H., Ogawa, T., Yoshimura, F., Otsuka, K., Kokeguchi, S., Kato, K., ... Kotani, S. (1988). Immunobiological activities of a porin fraction isolated from Fusobacterium nucleatum ATCC 10953. Infection and Immunity, 56(4), 855-863.

Immunobiological activities of a porin fraction isolated from Fusobacterium nucleatum ATCC 10953. / Takada, H.; Ogawa, T.; Yoshimura, F.; Otsuka, K.; Kokeguchi, Susumu; Kato, K.; Umemoto, T.; Kotani, S.

In: Infection and Immunity, Vol. 56, No. 4, 1988, p. 855-863.

Research output: Contribution to journalArticle

Takada, H, Ogawa, T, Yoshimura, F, Otsuka, K, Kokeguchi, S, Kato, K, Umemoto, T & Kotani, S 1988, 'Immunobiological activities of a porin fraction isolated from Fusobacterium nucleatum ATCC 10953', Infection and Immunity, vol. 56, no. 4, pp. 855-863.
Takada, H. ; Ogawa, T. ; Yoshimura, F. ; Otsuka, K. ; Kokeguchi, Susumu ; Kato, K. ; Umemoto, T. ; Kotani, S. / Immunobiological activities of a porin fraction isolated from Fusobacterium nucleatum ATCC 10953. In: Infection and Immunity. 1988 ; Vol. 56, No. 4. pp. 855-863.
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AU - Kokeguchi, Susumu

AU - Kato, K.

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AU - Kotani, S.

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