Immunoassay for human serum albumin using capillary electrophoresis-semiconductor laser-induced fluorometry

Shinichi Miki, Takashi Kaneta, Totaro Imasaka

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Capillary electrophoresis combined with semiconductor laser-induced fluorometry was applied to an immunoassay of human serum albumin. Human serum albumin was labeled with a fluorescent molecule (Cy5), which has an absorption maximum at 649 nm. The labeled albumin was purified by ultrafiltration in order to reduce signals, which are unreacted labeling reagent, product, and fragment products derived therefrom. After the purification, no signal for unreacted labeling reagent and fragment products was detectable in the electropherogram of the labeled albumin. The labeled albumin was then reacted with anti-albumin to form an immunocomplex, which was separated from the excess free albumin. The competitive immunoassay was used in the determination of human serum albumin in a controlled serum sample, using the labeled albumin. The obtained value was found to be 0.21±0.02 mg/ml, which is in good agreement with other known values.

Original languageEnglish
Pages (from-to)337-342
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume759
Issue number2
DOIs
Publication statusPublished - Aug 15 2001
Externally publishedYes

Keywords

  • Human serum albumin
  • Semi-conductor laser-induced fluorometry

ASJC Scopus subject areas

  • Chemistry(all)

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