Immunoadjuvant effects of polyadenylic: Polyuridylic acids through TLR3 and TLR7

Takahiro Sugiyama, Katsuaki Hoshino, Masuyoshi Saito, Takahiro Yano, Izumi Sasaki, Chihiro Yamazaki, Shizuo Akira, Tsuneyasu Kaisho

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Double-stranded RNA (dsRNA) is produced upon viral infection and can activate innate immunity. Polyinosinic:polycytidylic acids [poly(I:C)] is a synthetic mimetic of dsRNA and functions through an endosomal receptor, Toll-like receptor (TLR) 3 or cytosolic receptors. Another type of dsRNA, polyadenylic:polyuridylic acids [poly(A:U)], can also act as an immune adjuvant, but it remains unclear how it exhibits its adjuvant effects. Here, we have characterized the adjuvant effects of poly(A:U). Poly(A:U) could induce both IFN-α and IL-12p40 from murine bone marrow dendritic cells (DCs). Poly(A:U)-induced IFN-α production depended on a DC subset, plasmacytoid dendritic cell (pDC), and required TLR7. IL-12p40 was also produced by poly(A:U)-stimulated pDC in a TLR7-dependent manner. In addition to pDC, conventional dendritic cell (cDC) also produced IL-12p40 in response to poly(A:U). This IL-12p40 induction resulted from two cDC subsets, CD24high cDC and CD11bhigh cDC in a TLR3- and TLR7-dependent manner, respectively. In vivo injection of poly(A:U) with antigen led to clonal expansion of and IFN-γ production from antigen-specific CD8+ T cells. Consistent with the in vitro findings, TLR3 and TLR7 were required for the clonal T-cell expansion. Notably, TLR3, rather than TLR7, was critical for generating IFN-γ-producing CD8+ T cells. CD8+ T-cell responses induced by poly(A:U) were independent of type I IFN signaling. Our results demonstrate that poly(A:U) functions as an in vivo immunoadjuvant mainly through TLR3 and TLR7.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalInternational Immunology
Volume20
Issue number1
DOIs
Publication statusPublished - Jan 2008
Externally publishedYes

Fingerprint

Poly A-U
Immunologic Adjuvants
Dendritic Cells
Interleukin-12 Subunit p40
Double-Stranded RNA
T-Lymphocytes
Toll-Like Receptor 3
CD8 Antigens
Poly I-C
Virus Diseases
Innate Immunity
Bone Marrow Cells

Keywords

  • Dendritic cell
  • Double-stranded RNA
  • Immune adjuvant
  • Toll-like receptor

ASJC Scopus subject areas

  • Immunology

Cite this

Sugiyama, T., Hoshino, K., Saito, M., Yano, T., Sasaki, I., Yamazaki, C., ... Kaisho, T. (2008). Immunoadjuvant effects of polyadenylic: Polyuridylic acids through TLR3 and TLR7. International Immunology, 20(1), 1-9. https://doi.org/10.1093/intimm/dxm112

Immunoadjuvant effects of polyadenylic : Polyuridylic acids through TLR3 and TLR7. / Sugiyama, Takahiro; Hoshino, Katsuaki; Saito, Masuyoshi; Yano, Takahiro; Sasaki, Izumi; Yamazaki, Chihiro; Akira, Shizuo; Kaisho, Tsuneyasu.

In: International Immunology, Vol. 20, No. 1, 01.2008, p. 1-9.

Research output: Contribution to journalArticle

Sugiyama, T, Hoshino, K, Saito, M, Yano, T, Sasaki, I, Yamazaki, C, Akira, S & Kaisho, T 2008, 'Immunoadjuvant effects of polyadenylic: Polyuridylic acids through TLR3 and TLR7', International Immunology, vol. 20, no. 1, pp. 1-9. https://doi.org/10.1093/intimm/dxm112
Sugiyama, Takahiro ; Hoshino, Katsuaki ; Saito, Masuyoshi ; Yano, Takahiro ; Sasaki, Izumi ; Yamazaki, Chihiro ; Akira, Shizuo ; Kaisho, Tsuneyasu. / Immunoadjuvant effects of polyadenylic : Polyuridylic acids through TLR3 and TLR7. In: International Immunology. 2008 ; Vol. 20, No. 1. pp. 1-9.
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