Abstract
Problem: Laminins have important roles during placental and embryonic development. The aim of our study was to determine if active immunization of mice with laminin-1 could elicit an autoimmune response, and induce features of reproductive failure. Method of study: BALB/c mice were immunized with mouse laminin-1. Autoantibodies to laminin-1 were measured by enzyme-linked immunosorbent assay. Pregnant mice were killed on day 14 of pregnancy and examined for pregnancy outcome. Results: Mice immunized with laminin-1 developed elevated levels of anti-laminin-1 auto-antibodies contrary to the control group. A higher fetal resorption rate was found in the laminin-1 immunized group (23.8%) compared with that of the control group (12.2%), and was even higher in the subgroup of those animals with very high levels of anti-laminin-1 (P <0.01). Laminin-1 immunized mice also had lower fetal and placental weights. Conclusions: Active immunization with laminin-1 followed by elevated circulating anti-laminin-1 antibodies results in reproductive failure manifested by a higher fetal resorption rate.
| Original language | English |
|---|---|
| Pages (from-to) | 159-165 |
| Number of pages | 7 |
| Journal | American Journal of Reproductive Immunology |
| Volume | 50 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 2 2003 |
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Keywords
- Animal model
- Anti-laminin antibodies
- Autoantibodies
- Autoimmunity
- Fetal loss
- Laminin
ASJC Scopus subject areas
- Immunology
- Obstetrics and Gynaecology
Cite this
Immunization of naïve mice with mouse laminin-1 affected pregnancy outcome in a mouse model. / Matalon, Shelly Tartakover; Blank, Miri; Matsuura, Eiji; Inagaki, Junko; Nomizu, Motoyoshi; Levi, Yair; Koike, Takao; Shere, Yaniv; Ornoy, Asher; Shoenfeld, Yehuda.
In: American Journal of Reproductive Immunology, Vol. 50, No. 2, 02.08.2003, p. 159-165.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunization of naïve mice with mouse laminin-1 affected pregnancy outcome in a mouse model
AU - Matalon, Shelly Tartakover
AU - Blank, Miri
AU - Matsuura, Eiji
AU - Inagaki, Junko
AU - Nomizu, Motoyoshi
AU - Levi, Yair
AU - Koike, Takao
AU - Shere, Yaniv
AU - Ornoy, Asher
AU - Shoenfeld, Yehuda
PY - 2003/8/2
Y1 - 2003/8/2
N2 - Problem: Laminins have important roles during placental and embryonic development. The aim of our study was to determine if active immunization of mice with laminin-1 could elicit an autoimmune response, and induce features of reproductive failure. Method of study: BALB/c mice were immunized with mouse laminin-1. Autoantibodies to laminin-1 were measured by enzyme-linked immunosorbent assay. Pregnant mice were killed on day 14 of pregnancy and examined for pregnancy outcome. Results: Mice immunized with laminin-1 developed elevated levels of anti-laminin-1 auto-antibodies contrary to the control group. A higher fetal resorption rate was found in the laminin-1 immunized group (23.8%) compared with that of the control group (12.2%), and was even higher in the subgroup of those animals with very high levels of anti-laminin-1 (P <0.01). Laminin-1 immunized mice also had lower fetal and placental weights. Conclusions: Active immunization with laminin-1 followed by elevated circulating anti-laminin-1 antibodies results in reproductive failure manifested by a higher fetal resorption rate.
AB - Problem: Laminins have important roles during placental and embryonic development. The aim of our study was to determine if active immunization of mice with laminin-1 could elicit an autoimmune response, and induce features of reproductive failure. Method of study: BALB/c mice were immunized with mouse laminin-1. Autoantibodies to laminin-1 were measured by enzyme-linked immunosorbent assay. Pregnant mice were killed on day 14 of pregnancy and examined for pregnancy outcome. Results: Mice immunized with laminin-1 developed elevated levels of anti-laminin-1 auto-antibodies contrary to the control group. A higher fetal resorption rate was found in the laminin-1 immunized group (23.8%) compared with that of the control group (12.2%), and was even higher in the subgroup of those animals with very high levels of anti-laminin-1 (P <0.01). Laminin-1 immunized mice also had lower fetal and placental weights. Conclusions: Active immunization with laminin-1 followed by elevated circulating anti-laminin-1 antibodies results in reproductive failure manifested by a higher fetal resorption rate.
KW - Animal model
KW - Anti-laminin antibodies
KW - Autoantibodies
KW - Autoimmunity
KW - Fetal loss
KW - Laminin
UR - http://www.scopus.com/inward/record.url?scp=10744232539&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10744232539&partnerID=8YFLogxK
U2 - 10.1034/j.1600-0897.2003.00068.x
DO - 10.1034/j.1600-0897.2003.00068.x
M3 - Article
C2 - 12846680
AN - SCOPUS:10744232539
VL - 50
SP - 159
EP - 165
JO - American Journal of Reproductive Immunology and Microbiology
JF - American Journal of Reproductive Immunology and Microbiology
SN - 1046-7408
IS - 2
ER -