Immunization of naïve mice with mouse laminin-1 affected pregnancy outcome in a mouse model

Shelly Tartakover Matalon, Miri Blank, Eiji Matsuura, Junko Inagaki, Motoyoshi Nomizu, Yair Levi, Takao Koike, Yaniv Shere, Asher Ornoy, Yehuda Shoenfeld

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Problem: Laminins have important roles during placental and embryonic development. The aim of our study was to determine if active immunization of mice with laminin-1 could elicit an autoimmune response, and induce features of reproductive failure. Method of study: BALB/c mice were immunized with mouse laminin-1. Autoantibodies to laminin-1 were measured by enzyme-linked immunosorbent assay. Pregnant mice were killed on day 14 of pregnancy and examined for pregnancy outcome. Results: Mice immunized with laminin-1 developed elevated levels of anti-laminin-1 auto-antibodies contrary to the control group. A higher fetal resorption rate was found in the laminin-1 immunized group (23.8%) compared with that of the control group (12.2%), and was even higher in the subgroup of those animals with very high levels of anti-laminin-1 (P < 0.01). Laminin-1 immunized mice also had lower fetal and placental weights. Conclusions: Active immunization with laminin-1 followed by elevated circulating anti-laminin-1 antibodies results in reproductive failure manifested by a higher fetal resorption rate.

Original languageEnglish
Pages (from-to)159-165
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume50
Issue number2
DOIs
Publication statusPublished - Aug 2 2003

Keywords

  • Animal model
  • Anti-laminin antibodies
  • Autoantibodies
  • Autoimmunity
  • Fetal loss
  • Laminin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

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