TY - JOUR
T1 - Immunization of naïve mice with mouse laminin-1 affected pregnancy outcome in a mouse model
AU - Matalon, Shelly Tartakover
AU - Blank, Miri
AU - Matsuura, Eiji
AU - Inagaki, Junko
AU - Nomizu, Motoyoshi
AU - Levi, Yair
AU - Koike, Takao
AU - Shere, Yaniv
AU - Ornoy, Asher
AU - Shoenfeld, Yehuda
PY - 2003/8/2
Y1 - 2003/8/2
N2 - Problem: Laminins have important roles during placental and embryonic development. The aim of our study was to determine if active immunization of mice with laminin-1 could elicit an autoimmune response, and induce features of reproductive failure. Method of study: BALB/c mice were immunized with mouse laminin-1. Autoantibodies to laminin-1 were measured by enzyme-linked immunosorbent assay. Pregnant mice were killed on day 14 of pregnancy and examined for pregnancy outcome. Results: Mice immunized with laminin-1 developed elevated levels of anti-laminin-1 auto-antibodies contrary to the control group. A higher fetal resorption rate was found in the laminin-1 immunized group (23.8%) compared with that of the control group (12.2%), and was even higher in the subgroup of those animals with very high levels of anti-laminin-1 (P < 0.01). Laminin-1 immunized mice also had lower fetal and placental weights. Conclusions: Active immunization with laminin-1 followed by elevated circulating anti-laminin-1 antibodies results in reproductive failure manifested by a higher fetal resorption rate.
AB - Problem: Laminins have important roles during placental and embryonic development. The aim of our study was to determine if active immunization of mice with laminin-1 could elicit an autoimmune response, and induce features of reproductive failure. Method of study: BALB/c mice were immunized with mouse laminin-1. Autoantibodies to laminin-1 were measured by enzyme-linked immunosorbent assay. Pregnant mice were killed on day 14 of pregnancy and examined for pregnancy outcome. Results: Mice immunized with laminin-1 developed elevated levels of anti-laminin-1 auto-antibodies contrary to the control group. A higher fetal resorption rate was found in the laminin-1 immunized group (23.8%) compared with that of the control group (12.2%), and was even higher in the subgroup of those animals with very high levels of anti-laminin-1 (P < 0.01). Laminin-1 immunized mice also had lower fetal and placental weights. Conclusions: Active immunization with laminin-1 followed by elevated circulating anti-laminin-1 antibodies results in reproductive failure manifested by a higher fetal resorption rate.
KW - Animal model
KW - Anti-laminin antibodies
KW - Autoantibodies
KW - Autoimmunity
KW - Fetal loss
KW - Laminin
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U2 - 10.1034/j.1600-0897.2003.00068.x
DO - 10.1034/j.1600-0897.2003.00068.x
M3 - Article
C2 - 12846680
AN - SCOPUS:10744232539
VL - 50
SP - 159
EP - 165
JO - American Journal of Reproductive Immunology
JF - American Journal of Reproductive Immunology
SN - 1046-7408
IS - 2
ER -