Immunity to Rice black streaked dwarf virus, a plant reovirus, can be achieved in rice plants by RNA silencing against the gene for the viroplasm component protein

Takumi Shimizu, Eiko Nakazono-Nagaoka, Fusamichi Akita, Tamaki Uehara-Ichiki, Toshihiro Omura, Takahide Sasaya

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The nonstructural protein P9-1 of Rice black streaked dwarf virus has been confirmed to accumulate in viroplasms, the putative sites of viral replication, in infected plants and insects. We transformed rice plants by introducing an RNA interference construct against the P9-1-encoding gene. The resultant transgenic plants accumulated short interfering RNAs specific to the construct. All progenies produced by self-fertilization of these transgenic plants with induced RNA interference against the gene for P9-1 were resistant to infection by the virus. Our results demonstrated that interfering with the expression of a viroplasm component protein of plant reoviruses, which plays an important role in viral proliferation, might be a practical and effective way to control plant reovirus infection in crop plants.

Original languageEnglish
Pages (from-to)400-403
Number of pages4
JournalVirus Research
Volume160
Issue number1-2
DOIs
Publication statusPublished - Sep 2011
Externally publishedYes

Fingerprint

Plant RNA
Gene Components
Plant Viruses
RNA Interference
Immunity
Genetically Modified Plants
Reoviridae Infections
Self-Fertilization
Plant Structures
Plant Proteins
Proteins
Virus Diseases
Small Interfering RNA
Genes
Insects
Viruses
Oryza

Keywords

  • Fijivirus
  • Reoviridae
  • RNA interference
  • Transgenic rice
  • Viral resistance

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Immunity to Rice black streaked dwarf virus, a plant reovirus, can be achieved in rice plants by RNA silencing against the gene for the viroplasm component protein. / Shimizu, Takumi; Nakazono-Nagaoka, Eiko; Akita, Fusamichi; Uehara-Ichiki, Tamaki; Omura, Toshihiro; Sasaya, Takahide.

In: Virus Research, Vol. 160, No. 1-2, 09.2011, p. 400-403.

Research output: Contribution to journalArticle

Shimizu, Takumi ; Nakazono-Nagaoka, Eiko ; Akita, Fusamichi ; Uehara-Ichiki, Tamaki ; Omura, Toshihiro ; Sasaya, Takahide. / Immunity to Rice black streaked dwarf virus, a plant reovirus, can be achieved in rice plants by RNA silencing against the gene for the viroplasm component protein. In: Virus Research. 2011 ; Vol. 160, No. 1-2. pp. 400-403.
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