Immune response of post-transplant peripheral lymphocytes against the patient pre-B cell line, NAGL-1

Masanobu Kasai, Yoshiki Akatsuka, Nobuhiko Emi, Hirofumi Taji, Akio Kohno, Akihiro Abe, Mitsune Tanimoto, Yoshihisa Kodera, Hidehiko Saito

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Abstract

We have established a pre-B acute lymphoblastic leukemia (ALL) cell line, NAGL-1, from the bone marrow of a patient diagnosed with pre-B ALL. The patient has been disease-free for the 4 years since allogeneic bone marrow transplantation from her HLA-genotypically identical sister. NAGL-1 showed a pre-B cell phenotype (CD19+, CD10+, cμ+, sμ-) mostly identical to freshly isolated leukemic cells from the patient. This cell line strongly expressed HLA class I and HLA-DR molecules, as well as the costimulatory molecules CD54, CD40, and CD86. Cytotoxic T-lymphocyte (CTL) lines were generated by stimulating the donor-derived peripheral blood mononuclear cells with either irradiated leukemic cells or NAGL-1. Both CTL lines showed specific lysis against NAGL-1 in 51Cr release assays. Lytic activity was partially inhibited by anti-CD8 and anti-HLA class I monoclonal antibodies. Treatment of NAGL-1 with TNF-α increased its susceptibility to the CTL line. One CD8+ T cell clone derived from the CTL line killed both the patient phytohemagglutinin (PHA) blasts and NAGL-1 but not the donor PHA blasts, suggesting that the clone recognized the patient-specific minor antigen presented on both PHA blasts and NAGL-1. Utilization of leukemic cell lines could be a useful model for the development of CTL lines and clones for immuno-logical study and potential immunotherapy.

Original languageEnglish
Pages (from-to)112-118
Number of pages7
JournalInternational journal of hematology
Volume69
Issue number2
Publication statusPublished - 1999

Keywords

  • Graft-vs.-leukemia
  • NAGL-1
  • Pre-b cell leukemia

ASJC Scopus subject areas

  • Hematology

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  • Cite this

    Kasai, M., Akatsuka, Y., Emi, N., Taji, H., Kohno, A., Abe, A., Tanimoto, M., Kodera, Y., & Saito, H. (1999). Immune response of post-transplant peripheral lymphocytes against the patient pre-B cell line, NAGL-1. International journal of hematology, 69(2), 112-118.