Immortalization of human lymphocytes by co-cultivation with lethally irradiated autologous T-cell lines harbouring human T-cell leukaemia virus-I

T. Akagi; Y. Ohtsuki; K. Takahashi; I. Takeda; T. Oka; I. Miyoshi

doi:10.1007/BF00402508

Immortalization of human lymphocytes by co-cultivation with lethally irradiated autologous T-cell lines harbouring human T-cell leukaemia virus-I

T. Akagi, Y. Ohtsuki, K. Takahashi, I. Takeda, T. Oka, I. Miyoshi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Human adult peripheral blood lymphocytes were successfully immortalized by co-cultivation with irradiated autologous and homologous T-cell lines harbouring human T-cell leukaemia virus-I (HTLV-I). The efficiency of transformation was the same in both cases. The participation of alloantigen stimulation in co-cultivation procedures is discussed, and it is stressed that factors other than alloantigen stimulation might be required for the efficient immortalization of human T-lymphocytes in vitro by HTLV.

Original language	English
Pages (from-to)	82-84
Number of pages	3
Journal	Journal of cancer research and clinical oncology
Volume	110
Issue number	1
DOIs	https://doi.org/10.1007/BF00402508
Publication status	Published - Jul 1 1985
Externally published	Yes

Keywords

Human T-cell leukaemia virus
Lymphocyte
Transformation

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1007/BF00402508

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Immortalization of human lymphocytes by co-cultivation with lethally irradiated autologous T-cell lines harbouring human T-cell leukaemia virus-I. / Akagi, T.; Ohtsuki, Y.; Takahashi, K.; Takeda, I.; Oka, T.; Miyoshi, I.

In: Journal of cancer research and clinical oncology, Vol. 110, No. 1, 01.07.1985, p. 82-84.

Research output: Contribution to journal › Article › peer-review

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AU - Akagi, T.

AU - Ohtsuki, Y.

AU - Takahashi, K.

AU - Takeda, I.

AU - Oka, T.

AU - Miyoshi, I.

PY - 1985/7/1

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N2 - Human adult peripheral blood lymphocytes were successfully immortalized by co-cultivation with irradiated autologous and homologous T-cell lines harbouring human T-cell leukaemia virus-I (HTLV-I). The efficiency of transformation was the same in both cases. The participation of alloantigen stimulation in co-cultivation procedures is discussed, and it is stressed that factors other than alloantigen stimulation might be required for the efficient immortalization of human T-lymphocytes in vitro by HTLV.

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KW - Lymphocyte

KW - Transformation

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Immortalization of human lymphocytes by co-cultivation with lethally irradiated autologous T-cell lines harbouring human T-cell leukaemia virus-I

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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