Immobilization of surface non-affinitive protein onto a metal surface by an external electric field

Olivier Tramis, Ryosuke Iizuka, Hajime Nakao, Hiroyuki Imanaka, Naoyuki Ishida, Koreyoshi Imamura

Research output: Contribution to journalArticle

Abstract

We investigated an alternate technique to coat the surface with a protein having no surface affinity, without the use of any exotic chemical agents. An external electric field was utilized to prepare the protein coating on a metal substrate. Stainless steel (St) substrate and lysozyme (LSZ) were used as the surface to be coated and the model non-adsorptive protein, respectively. Dynamics of the adsorption of LSZ on the St surface in the presence and absence of an external electric potential (EEP) were monitored by in-situ ellipsometry. Applying negative surface potential (−0.4 V vs Ag/AgCl) forced the adsorption of LSZ onto the St surface where LSZ did not adsorb without applying any EEP. The repetition of the EEP-application and -cut-off indicated the controllability of the LSZ coating amount depending on the total duration of the EEP-application. The coated LSZ largely remained bound to the surface even by the cut-off of the external electric field, the ratio of which to the detached amount was roughly constant (approximately 7:3). Furthermore, the LSZ coated surface on the St substrate was found to be reversibly switched between being affinitive and non-affinitive to a typical model protein adsorbate (bovine serum albumin) by the EEP-application and cut-off.

Original languageEnglish
Pages (from-to)348-353
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume129
Issue number3
DOIs
Publication statusPublished - Mar 2020

Keywords

  • Adsorption
  • Electrostatic interaction
  • Ellipsometer
  • External electric potential
  • Lysozyme
  • Metal oxide
  • Surface hydroxyl group

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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