Immobilization of intact liposomes on solid surfaces: A quartz crystal microbalance study

Thi Huong Vu, Toshinori Shimanouchi, Haruyuki Ishii, Hiroshi Umakoshi, Ryoichi Kuboi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Immobilization of model cell membranes (liposomes) on sensing devices is useful for the detection, evaluation, and analysis of interaction between cell membranes and proteins or other biological materials. The method to immobilize the liposomes on the solid surface was investigated by using the quartz crystal microbalance (QCM) method, focusing on the density of immobilized liposomes and their stability. The different liposomes were applied onto the planar surface of the QCM electrode by varying the immobilization method. The results showed that the immobilized liposomes depended not only on the immobilization method but also on the properties of the liposomes. The liposome with low membrane fluidity likely showed intact immobilization on a solid surface. An electrostatic interaction also affected the amount of immobilized liposomes on the functionalized quartz crystal. By using the amino-coupling method, intact liposomes could be immobilized on a solid surface and immobilized liposomes could stabilize for 10 h with frequency changes less than 5%.

Original languageEnglish
Pages (from-to)902-907
Number of pages6
JournalJournal of Colloid and Interface Science
Volume336
Issue number2
DOIs
Publication statusPublished - Aug 15 2009
Externally publishedYes

Fingerprint

Liposomes
Quartz crystal microbalances
Cell membranes
Quartz
Fluidity
Coulomb interactions
Biological materials
Membrane Proteins
Proteins
Membranes
Crystals
Electrodes

Keywords

  • Amino coupling
  • Immobilized liposome
  • Membrane fluidity
  • Quartz crystal microbalance

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Colloid and Surface Chemistry

Cite this

Immobilization of intact liposomes on solid surfaces : A quartz crystal microbalance study. / Vu, Thi Huong; Shimanouchi, Toshinori; Ishii, Haruyuki; Umakoshi, Hiroshi; Kuboi, Ryoichi.

In: Journal of Colloid and Interface Science, Vol. 336, No. 2, 15.08.2009, p. 902-907.

Research output: Contribution to journalArticle

Vu, Thi Huong ; Shimanouchi, Toshinori ; Ishii, Haruyuki ; Umakoshi, Hiroshi ; Kuboi, Ryoichi. / Immobilization of intact liposomes on solid surfaces : A quartz crystal microbalance study. In: Journal of Colloid and Interface Science. 2009 ; Vol. 336, No. 2. pp. 902-907.
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