Immediate differentiation of neuronal cells from stem/progenitor-like cells in the avian iris tissues

Tamami Matsushita, Ai Fujihara, Lars Royall, Satoshi Kagiwada, Mitsuko Kosaka, Masasuke Araki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A simple culture method that was recently developed in our laboratory was applied to the chick iris tissues to characterize neural stem/progenitor-like cells. Iris tissue is a non-neuronal tissue and does not contain any neuronal cells. In the present study we isolated iris tissues from chick embryos just prior to hatching. The isolated iris pigmented epithelium (IPE) or the stroma was embedded in Matrigel and cultured in Dulbecco's MEM supplemented with either fetal bovine serum or the synthetic serum replacement solution B27. Within 24h of culture, elongated cells with long processes extended out from the explants of both tissues and were positively stained for various neuronal markers such as transitin, Tuj-1 and acetylated tubulin. After a longer culture period, cells positive for photoreceptor markers like rhodopsin, iodopsin and visinin were found, suggesting that the iris tissues contain retinal stem/progenitor-like cells. Several growth factors were examined to determine their effects on neuronal differentiation. EGF was shown to dramatically enhance neuronal cell differentiation, particularly the elongation of neuronal fibers. The addition of exogenous FGF2, however, did not show any positive effects on neuronal differentiation, although FGF signaling inhibitor, SU5402, suppressed neuronal differentiation. The results show that neuronal stem/progenitor-like cells can differentiate into neuronal cells immediately after they are transferred into an appropriate environment. This process did not require any exogenous factors, suggesting that neural stem/progenitor-like cells are simply suppressed from neuronal differentiation within the tissue, and isolation from the tissue releases the cells from the suppression mechanism.

Original languageEnglish
Pages (from-to)16-26
Number of pages11
JournalExperimental Eye Research
Volume123
DOIs
Publication statusPublished - 2014

Fingerprint

Iris
Cell Differentiation
Stem Cells
Cell Culture Techniques
Photoreceptor Cells
Rhodopsin
Fibroblast Growth Factor 2
Chick Embryo
Tubulin
Serum
Epidermal Growth Factor
Intercellular Signaling Peptides and Proteins
Epithelium

Keywords

  • Extracellular matrix
  • Iris
  • Neural stem/progenitor
  • Tissue culture

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Immediate differentiation of neuronal cells from stem/progenitor-like cells in the avian iris tissues. / Matsushita, Tamami; Fujihara, Ai; Royall, Lars; Kagiwada, Satoshi; Kosaka, Mitsuko; Araki, Masasuke.

In: Experimental Eye Research, Vol. 123, 2014, p. 16-26.

Research output: Contribution to journalArticle

Matsushita, Tamami ; Fujihara, Ai ; Royall, Lars ; Kagiwada, Satoshi ; Kosaka, Mitsuko ; Araki, Masasuke. / Immediate differentiation of neuronal cells from stem/progenitor-like cells in the avian iris tissues. In: Experimental Eye Research. 2014 ; Vol. 123. pp. 16-26.
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