Imbalance of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandibular glands

Daisuke Ekuni, Yasumasa Endo, Koichiro Irie, Tetsuji Azuma, Naofumi Tamaki, Takaaki Tomofuji, Manabu Morita

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: Epidemiologic studies suggest a relationship between periodontitis and salivary gland dysfunction. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and pathological changes of submandibular glands. Design: Fourteen male Wistar rats (8 weeks old) were divided into two groups (n = 7/group): a control group and periodontitis group. Periodontitis was induced by ligature placement around the mandibular first molars. Serum levels for reactive oxygen metabolites, anti-oxidant and tumour necrosis factor (TNF)-α were measured at baseline, 2 and 4 weeks. At 4 weeks, the levels of 8-hydroxydeoxyguanosine were determined to evaluate oxidative damage of submandibular glands. Expression of TNF-α mRNA and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) as well as histological findings were also evaluated in the submandibular glands. Results: The rats with experimental periodontitis showed increase in the levels of serum reactive oxygen metabolites and TNF-α, and a decrease of anti-oxidant power in a time-dependent manner. At 4 weeks, these rats also had significantly increased levels of 8-hydroxydeoxyguanosine and TNF-α, and increased number of TUNEL-positive cells and vacuolisation in the submandibular glands compared to the control rats. Conclusions: Imbalance of circulating oxidative/anti-oxidative status may be involved in vacuolisation and apoptosis of submandibular glands in the rat periodontitis model.

Original languageEnglish
Pages (from-to)170-176
Number of pages7
JournalArchives of Oral Biology
Volume55
Issue number2
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Submandibular Gland
Periodontitis
Apoptosis
Tumor Necrosis Factor-alpha
Oxidants
Oxygen
DNA Nucleotidylexotransferase
Transferases
Salivary Glands
Serum
Ligation
Wistar Rats
Epidemiologic Studies
Control Groups
Messenger RNA

Keywords

  • Apoptosis
  • Oxidative stress
  • Periodontal diseases
  • Submandibular gland

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Imbalance of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandibular glands. / Ekuni, Daisuke; Endo, Yasumasa; Irie, Koichiro; Azuma, Tetsuji; Tamaki, Naofumi; Tomofuji, Takaaki; Morita, Manabu.

In: Archives of Oral Biology, Vol. 55, No. 2, 02.2010, p. 170-176.

Research output: Contribution to journalArticle

Ekuni, Daisuke ; Endo, Yasumasa ; Irie, Koichiro ; Azuma, Tetsuji ; Tamaki, Naofumi ; Tomofuji, Takaaki ; Morita, Manabu. / Imbalance of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandibular glands. In: Archives of Oral Biology. 2010 ; Vol. 55, No. 2. pp. 170-176.
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