Imaging of transgenic cricket embryos reveals cell movements consistent with a syncytial patterning mechanism

Taro Nakamura, Masato Yoshizaki, Shotaro Ogawa, Haruko Okamoto, Yohei Shinmyo, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji, Taro Mito

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The mode of insect embryogenesis varies among species, reflecting adaptations to different life history strategies [1, 2]. In holometabolous insects, which include the model systems, such as the fruit fly and the red flour beetle, a large proportion of the blastoderm produces an embryo, whereas hemimetabolous embryos generally arise from a small region of the blastoderm [3]. Despite their importance in evolutionary studies, information of early developmental dynamics of hemimetabolous insects remains limited. Here, to clarify how maternal and gap gene products act in patterning the embryo of basal hemimetabolous insects, we analyzed the dynamic segmentation process in transgenic embryos of an intermediate-germ insect species, the cricket, Gryllus bimaculatus. Our data based on live imaging of fluorescently labeled embryonic cells and nuclei suggest that the positional specification of the cellular blastoderm may be established in the syncytium, where maternally derived gradients could act fundamentally in a way that is similar to that of Drosophila, namely throughout the egg. Then, the blastoderm cells move dynamically, retaining their positional information to form the posteriorly localized germ anlage. Furthermore, we find that the anterior head region of the cricket embryo is specified by orthodenticle in a cellular environment earlier than the gnathal and thoracic regions. Our findings imply that the syncytial mode of the early segmentation in long-germ insects evolved from a dynamic syncytial-to-cellular mode found in the present study, accompanied by a heterochronic shift of gap gene action.

Original languageEnglish
Pages (from-to)1641-1647
Number of pages7
JournalCurrent Biology
Volume20
Issue number18
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Gryllidae
cell movement
Blastoderm
Cell Movement
Insects
blastoderm
embryo (animal)
Embryonic Structures
Cells
image analysis
genetically modified organisms
Imaging techniques
insects
Genes
Fruits
Specifications
Gryllus bimaculatus
Tribolium castaneum
Beetles
giant cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Imaging of transgenic cricket embryos reveals cell movements consistent with a syncytial patterning mechanism. / Nakamura, Taro; Yoshizaki, Masato; Ogawa, Shotaro; Okamoto, Haruko; Shinmyo, Yohei; Bando, Tetsuya; Ohuchi, Hideyo; Noji, Sumihare; Mito, Taro.

In: Current Biology, Vol. 20, No. 18, 2010, p. 1641-1647.

Research output: Contribution to journalArticle

Nakamura, Taro ; Yoshizaki, Masato ; Ogawa, Shotaro ; Okamoto, Haruko ; Shinmyo, Yohei ; Bando, Tetsuya ; Ohuchi, Hideyo ; Noji, Sumihare ; Mito, Taro. / Imaging of transgenic cricket embryos reveals cell movements consistent with a syncytial patterning mechanism. In: Current Biology. 2010 ; Vol. 20, No. 18. pp. 1641-1647.
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