Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node

Masahiro Kitano, Chihiro Yamazaki, Akiko Takumi, Takashi Ikeno, Hiroaki Hemmi, Noriko Takahashi, Kanako Shimizu, Scott E. Fraser, Katsuaki Hoshino, Tsuneyasu Kaisho, Takaharu Okada

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Dendritic cells (DCs) are antigen-presenting cells specialized for activating T cells to elicit effector T-cell functions. Cross-presenting DCs are a DC subset capable of presenting antigens to CD8+ T cells and play critical roles in cytotoxic T-cell-mediated immune responses to microorganisms and cancer. Although their importance is known, the spatiotemporal dynamics of cross-presenting DCs in vivo are incompletely understood. Here, we study the T-cell zone in skin-draining lymph nodes (SDLNs) and find it is compartmentalized into regions for CD8+ T-cell activation by cross-presenting DCs that express the chemokine (C motif) receptor 1 gene, Xcr1 and for CD4+ T-cell activation by CD11b+ DCs. Xcr1-expressing DCs in the SDLNs are composed of two different populations: migratory (CD103hi) DCs, which immigrate from the skin, and resident (CD8αhi) DCs, which develop in the nodes. To characterize the dynamic interactions of these distinct DC populations with CD8+ T cells during their activation in vivo, we developed a photoconvertible reporter mouse strain, which permits us to distinctively visualize the migratory and resident subsets of Xcr1-expressing DCs. After leaving the skin, migratory DCs infiltrated to the deep T-cell zone of the SDLNs over 3 d, which corresponded to their half-life in the SDLNs. Intravital two-photon imaging showed that after soluble antigen immunization, the newly arriving migratory DCs more efficiently form sustained conjugates with antigen-specific CD8+ T cells than other Xcr1-expressing DCs in the SDLNs. These results offer in vivo evidence for differential contributions of migratory and resident cross-presenting DCs to CD8+ T-cell activation.

Original languageEnglish
Pages (from-to)1044-1049
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number4
DOIs
Publication statusPublished - Jan 26 2016

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Dendritic Cells
Lymph Nodes
Skin
T-Lymphocytes
Langerhans Cells
CD8 Antigens
C Chemokines
Antigen-Presenting Cells
Photons
Population
Half-Life
Immunization
Antigens

Keywords

  • CD8 T cell
  • Cross-presentation
  • Dendritic cell
  • Intravital two-photon imaging
  • Photoconversion

ASJC Scopus subject areas

  • General

Cite this

Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node. / Kitano, Masahiro; Yamazaki, Chihiro; Takumi, Akiko; Ikeno, Takashi; Hemmi, Hiroaki; Takahashi, Noriko; Shimizu, Kanako; Fraser, Scott E.; Hoshino, Katsuaki; Kaisho, Tsuneyasu; Okada, Takaharu.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 4, 26.01.2016, p. 1044-1049.

Research output: Contribution to journalArticle

Kitano, M, Yamazaki, C, Takumi, A, Ikeno, T, Hemmi, H, Takahashi, N, Shimizu, K, Fraser, SE, Hoshino, K, Kaisho, T & Okada, T 2016, 'Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 4, pp. 1044-1049. https://doi.org/10.1073/pnas.1513607113
Kitano, Masahiro ; Yamazaki, Chihiro ; Takumi, Akiko ; Ikeno, Takashi ; Hemmi, Hiroaki ; Takahashi, Noriko ; Shimizu, Kanako ; Fraser, Scott E. ; Hoshino, Katsuaki ; Kaisho, Tsuneyasu ; Okada, Takaharu. / Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 4. pp. 1044-1049.
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