Imaging of Molecular Interaction Between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes

Mitsuhiro Hoshijima, Eriko Aoyama, Hiroshi Kamioka, Masaharu Takigawa

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

An in situ proximity ligation assay (PLA) enables visualization of protein interactions in fixed cells. It is a powerful method for investigating protein-protein binding of endogenously expressed proteins. To confirm binding between CCN2 and Rab14 GTPase (Rab14) in chondrocytes, we performed a PLA using chondrocytic HCS-2/8 cells. The protocol in this chapter introduces an optimized technique for visualizing intracellular interactions of CCN2 and Rab14 in fixed cells using a PLA.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages31-37
Number of pages7
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2582
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • CCN2
  • Proximity ligation assay (PLA)
  • Rab14

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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