Illumination accelerates the decay of the O-intermediate of pharaonis phoborhodopsin (sensory rhodopsin II)

Masayuki Iwamoto, Yuki Sudo, Kazumi Shimono, Naoki Kamo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

pharaonis phoborhodopsin (ppR, also called pharaonis sensory rhodopsin II [psRII]) is a member of the archaeal rhodopsin family and acts as a repellent phototaxis receptor of Natronobacterium pharaonis. Upon illumination, ppR is excited and undergoes a linear cyclic photoreaction, namely, a photocycle that constitutes photointermediates such as M- and O-intermediates (ppRM and ppRO, respectively). Under a constant background illumination (>600 nm) that irradiates ppRO, the decay rate of the flash-induced ppRO increased with an increase in the background light intensity, indicating the photoreactivity of ppRO. Azide did not influence the light-accelerated ppRO decay, but the time required for the cycle to be completed became shortened in an azide concentration-dependent manner because of acceleration of ppRM decay. Hence, the turnover rate of photocycling increased appreciably in the presence of both the background illumination and the azide. The observation reported previously (Schmies, G. et al. 2000, Biophys. J. 78:967-976) is discussed in connection with the present observations.

Original languageEnglish
Pages (from-to)462-466
Number of pages5
JournalPhotochemistry and Photobiology
Volume76
Issue number4
DOIs
Publication statusPublished - Oct 1 2002
Externally publishedYes

Fingerprint

Sensory Rhodopsins
Azides
Lighting
illumination
Natronobacterium
Microbial Rhodopsins
decay
Photoreactivity
Light
luminous intensity
decay rates
flash
Observation
cycles

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Illumination accelerates the decay of the O-intermediate of pharaonis phoborhodopsin (sensory rhodopsin II). / Iwamoto, Masayuki; Sudo, Yuki; Shimono, Kazumi; Kamo, Naoki.

In: Photochemistry and Photobiology, Vol. 76, No. 4, 01.10.2002, p. 462-466.

Research output: Contribution to journalArticle

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