IL-8 is involved in homologous TNFα-, but not in IL-1β-induced neutrophil infiltration in rabbits

K. Miyamoto, A. Matsukawa, S. Ohkawara, K. Takagi, M. Yoshinaga

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective and Design: We examined the involvement of IL-8 in TNFα and IL-1β neutrophil infiltration, using homologous reagents. Subjects: Female New Zealand white rabbits (n = 211). Methods: After injection of TNFα (1 μg) or rrIL-1β (300 pg) into rabbit knee joints, the leukocyte influx and the production of IL-8 were estimated. Anti-TNFα mAb, rrIL-1Ra, and anti-IL-8 IgG (10 μg each) were administrated simultaneously with either TNFα or IL-1β. Results: Administration of TNFα or IL-1β induced IL-8 production; both of which peaked at 1 h after the injection. Maximum neutrophil infiltration induced by TNFα was inhibited by 38-56% by anti-IL-8 IgG, 22-43% by rrIL-1Ra, and 77-83% by anti-IL-8 IgG plus rrIL-1Ra. In contrast, maximum neutrophil infiltration induced by IL-1β was not inhibited by anti-IL-8 IgG whereas anti-TNFα mAb reduced the infiltration by 68-75%. Conclusions: The involvement of IL-8 in TNFα- and IL-1β-induced neutrophil infiltration is limited. Factor(s) other than IL-8 appear to function as major neutrophil chemoattractants.

Original languageEnglish
Pages (from-to)472-477
Number of pages6
JournalInflammation Research
Volume46
Issue number11
DOIs
Publication statusPublished - Dec 9 1997
Externally publishedYes

Keywords

  • IL-1 receptor antagonist
  • IL-1β
  • IL-8
  • Neutrophil infiltration
  • TNFα

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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