We investigated the involvement of IL-8 in the delayed vascular permeability (VP) in rabbit lipopolysaccharide (LPS)-pleurisy. Maximal level of interleukin-8 (IL-8) was detected in pleural fluid at 2 h after LPS injection and anti-IL-8 inhibited the delayed VP by 90%. Injection of homologous IL-8 induced VP, the time-course of which preceded that of LPS- induced delayed VP. Production of IL-8 in LPS-pleurisy was inhibited with antitumor necrosis factor at (TNF-α), whereas the production of TNF-α was not affected with anti-IL-8. Injection of IL-8 did not induce TNF-α production and anti-TNF-α had no effect on IL-8-induced VP. Injection of homologous TNF-α induced IL-8 production and VP, and TNF-α-induced delayed VP was blocked with anti-IL-8. These results indicate important roles of IL- 8 in LPS-induced delayed VP and that TNF-α causes the delayed VP through the production of IL-8.
|Number of pages||7|
|Journal||Journal of Leukocyte Biology|
|Publication status||Published - May 1998|
- Neutrophil infiltration
- Tumor necrosis factor α
ASJC Scopus subject areas
- Cell Biology