TY - JOUR
T1 - IL-8 is an essential mediator of the increased delayed-phase vascular permeability in LPS-induced rabbit pleurisy
AU - Fukumoto, Takumi
AU - Matsukawa, Akihiro
AU - Yoshimura, Teizo
AU - Edamitsu, Sunao
AU - Ohkawara, Susumu
AU - Takagi, Katsumasa
AU - Yoshinaga, Masaru
PY - 1998/5
Y1 - 1998/5
N2 - We investigated the involvement of IL-8 in the delayed vascular permeability (VP) in rabbit lipopolysaccharide (LPS)-pleurisy. Maximal level of interleukin-8 (IL-8) was detected in pleural fluid at 2 h after LPS injection and anti-IL-8 inhibited the delayed VP by 90%. Injection of homologous IL-8 induced VP, the time-course of which preceded that of LPS- induced delayed VP. Production of IL-8 in LPS-pleurisy was inhibited with antitumor necrosis factor at (TNF-α), whereas the production of TNF-α was not affected with anti-IL-8. Injection of IL-8 did not induce TNF-α production and anti-TNF-α had no effect on IL-8-induced VP. Injection of homologous TNF-α induced IL-8 production and VP, and TNF-α-induced delayed VP was blocked with anti-IL-8. These results indicate important roles of IL- 8 in LPS-induced delayed VP and that TNF-α causes the delayed VP through the production of IL-8.
AB - We investigated the involvement of IL-8 in the delayed vascular permeability (VP) in rabbit lipopolysaccharide (LPS)-pleurisy. Maximal level of interleukin-8 (IL-8) was detected in pleural fluid at 2 h after LPS injection and anti-IL-8 inhibited the delayed VP by 90%. Injection of homologous IL-8 induced VP, the time-course of which preceded that of LPS- induced delayed VP. Production of IL-8 in LPS-pleurisy was inhibited with antitumor necrosis factor at (TNF-α), whereas the production of TNF-α was not affected with anti-IL-8. Injection of IL-8 did not induce TNF-α production and anti-TNF-α had no effect on IL-8-induced VP. Injection of homologous TNF-α induced IL-8 production and VP, and TNF-α-induced delayed VP was blocked with anti-IL-8. These results indicate important roles of IL- 8 in LPS-induced delayed VP and that TNF-α causes the delayed VP through the production of IL-8.
KW - Inflammation
KW - Neutrophil infiltration
KW - Tumor necrosis factor α
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U2 - 10.1002/jlb.63.5.584
DO - 10.1002/jlb.63.5.584
M3 - Article
C2 - 9581802
AN - SCOPUS:0031946897
SN - 0741-5400
VL - 63
SP - 584
EP - 590
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 5
ER -