IL-21 inhibits IL-17A-producing γδ T-cell response after infection with Bacillus Calmette-Guérin via induction of apoptosis

Yinxia Huang, Yumiko Matsumura, Shinya Hatano, Naoto Noguchi, Tesshin Murakami, Yoichiro Iwakura, Xun Sun, Naoya Oohara, Yasunobu Yoshikai

Research output: Contribution to journalArticle

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Abstract

Innate γδ T cells expressing Vγ6 produce IL-17A at an early stage following infection with Mycobacterium bovis Bacillus Calmette-Guérin (BCG). In this study, we used IL-21 receptor knockout (IL-21R KO) mice and IL-21-producing recombinant BCG mice (rBCG-Ag85B-IL-21) to examine the role of IL-21 in the regulation of IL-17A-producing innate γδ T-cell response following BCG infection. IL-17A-producing Vγ6+ γδ T cells increased in the peritoneal cavity of IL-21R KO mice more than in wild type mice after BCG infection. In contrast, the number of IL-17A-producing Vγ6+ γδ T cells was significantly lower after inoculation with rBCG-Ag85B-IL-21 compared with control rBCG-Ag85B. Notably, exogenous IL-21 selectively induced apoptosis of IL-17A-producing Vγ6+ γδ T cells via Bim. Thus, these results suggest that IL-21 acts as a potent inhibitor of a IL-17A-producing γδ T-cell subset during BCG infection.

Original languageEnglish
Pages (from-to)588-597
Number of pages10
JournalInnate Immunity
Volume22
Issue number8
DOIs
Publication statusPublished - Nov 1 2016

Fingerprint

Interleukin-17
Bacillus
Apoptosis
T-Lymphocytes
Interleukin-21 Receptors
Infection
Knockout Mice
Peritoneal Cavity
T-Lymphocyte Subsets
Mycobacterium bovis
interleukin-21

Keywords

  • apoptosis
  • BCG
  • IL-17A
  • IL-21
  • γδ T cells

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases

Cite this

Huang, Y., Matsumura, Y., Hatano, S., Noguchi, N., Murakami, T., Iwakura, Y., ... Yoshikai, Y. (2016). IL-21 inhibits IL-17A-producing γδ T-cell response after infection with Bacillus Calmette-Guérin via induction of apoptosis. Innate Immunity, 22(8), 588-597. https://doi.org/10.1177/1753425916664125

IL-21 inhibits IL-17A-producing γδ T-cell response after infection with Bacillus Calmette-Guérin via induction of apoptosis. / Huang, Yinxia; Matsumura, Yumiko; Hatano, Shinya; Noguchi, Naoto; Murakami, Tesshin; Iwakura, Yoichiro; Sun, Xun; Oohara, Naoya; Yoshikai, Yasunobu.

In: Innate Immunity, Vol. 22, No. 8, 01.11.2016, p. 588-597.

Research output: Contribution to journalArticle

Huang, Y, Matsumura, Y, Hatano, S, Noguchi, N, Murakami, T, Iwakura, Y, Sun, X, Oohara, N & Yoshikai, Y 2016, 'IL-21 inhibits IL-17A-producing γδ T-cell response after infection with Bacillus Calmette-Guérin via induction of apoptosis', Innate Immunity, vol. 22, no. 8, pp. 588-597. https://doi.org/10.1177/1753425916664125
Huang, Yinxia ; Matsumura, Yumiko ; Hatano, Shinya ; Noguchi, Naoto ; Murakami, Tesshin ; Iwakura, Yoichiro ; Sun, Xun ; Oohara, Naoya ; Yoshikai, Yasunobu. / IL-21 inhibits IL-17A-producing γδ T-cell response after infection with Bacillus Calmette-Guérin via induction of apoptosis. In: Innate Immunity. 2016 ; Vol. 22, No. 8. pp. 588-597.
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