IL-17A promotes vascular calcification in an ex vivo murine aorta culture

Sumie Hiramatsu-Asano, Tomoyuki Mukai, Takahiko Akagi, Haruhito A. Uchida, Shunichi Fujita, Kazuhisa Nakano, Yoshitaka Morita

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Vascular calcification is characterized by mineral deposition in the vasculature, which is triggered by chronic systemic inflammation, including psoriasis. Psoriasis is an IL-17A-mediated inflammatory skin disease that is associated with exacerbated vascular calcification and high cardiovascular mortality. Although previous studies have shown that IL-17A induces vascular dysfunction in murine psoriasis models, it has not been clarified whether IL-17A induces vascular calcification. In this study, we investigated the potential vascular calcification-inducing effect of IL-17A in an ex vivo culture system. Methods: Thoracic and abdominal aortas from mice were cultured in a medium supplemented with inorganic phosphate and were treated with inflammatory cytokines (IL-1β, TNF-α, IL-6, and IL-17A). Vascular calcification was determined using micro-computed tomography (CT) and histological analyses. Results: IL-1β, TNF-α, and IL-6 did not significantly promote vascular calcification, whereas IL-17A significantly accelerated vascular calcification of the aorta, as indicated by the increased mineralized volume based on micro-CT analysis. Micro-CT and histological analyses also revealed that the promoting effect of IL-17A on vascular calcification was concentration dependent. Conclusions: IL-17A significantly promoted vascular calcification in ex vivo cultured aortas, which suggests that this mechanism is involved in the increased risk of cardiovascular events in IL-17A-mediated inflammatory diseases.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume604
DOIs
Publication statusPublished - May 14 2022
Externally publishedYes

Keywords

  • Aorta
  • Chronic inflammation
  • Ex vivo culture
  • IL-17A
  • Vascular calcification

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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