IL-10-treated dendritic cells decrease airway hyperresponsiveness and airway inflammation in mice

Toshiyuki Koya, Hiroyuki Matsuda, Katsuyuki Takeda, Shigeki Matsubara, Nobuaki Miyahara, Annette Balhorn, Azzeddine Dakhama, Erwin W. Gelfand

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Background: IL-10 affects dendritic cell (DC) function, but the effects on airway hyperresponsiveness (AHR) and inflammation are not defined. Objective: We sought to determine the importance of IL-10 in regulating DC function in allergen-induced AHR and airway inflammation. Methods: DCs were generated from bone marrow in the presence or absence of IL-10. In vivo IL-10-treated DCs from IL-10+/+ and IL-10-/- donors pulsed with ovalbumin (OVA) were transferred to naive or sensitized mice before challenge. In recipient mice AHR, cytokine levels, cell composition of bronchoalveolar lavage (BAL) fluid, and lung histology were monitored. Results: In vitro, IL-10-treated DCs expressed lower levels of CD11c, CD80, and CD86; expressed lower levels of IL-12; and suppressed TH2 cytokine production. In vivo, after transfer of OVA-pulsed IL-10-treated DCs, naive mice did not have AHR, airway eosinophilia, TH2 cytokine increase in BAL fluid, or goblet cell metaplasia when challenged, and in sensitized and challenged mice IL-10-treated DCs suppressed these responses. Levels of IL-10 in BAL fluid and numbers of lung CD4+IL-10+ T cells were increased in mice that received OVA-pulsed IL-10-treated DCs. Transfer of IL-10-treated DCs from IL-10-deficient mice were ineffective in suppressing the responses in sensitized and challenged mice. Conclusions: These data demonstrate that IL-10-treated DCs are potent suppressors of the development of AHR, inflammation, and TH2 cytokine production; these regulatory functions are at least in part through the induction of endogenous (DC) production of IL-10. Clinical implications: Modification of DC function by IL-10 can attenuate lung allergic responses, including the development of AHR.

Original languageEnglish
Pages (from-to)1241-1250
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Volume119
Issue number5
DOIs
Publication statusPublished - May 1 2007
Externally publishedYes

Keywords

  • IL-10-treated dendritic cells
  • airway hyperresponsiveness
  • eosinophils
  • regulatory T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'IL-10-treated dendritic cells decrease airway hyperresponsiveness and airway inflammation in mice'. Together they form a unique fingerprint.

Cite this