IGF-1 Gene Transfer to Human Synovial MSCs Promotes Their Chondrogenic Differentiation Potential without Induction of the Hypertrophic Phenotype

Yasutoshi Ikeda, Morito Sakaue, Ryota Chijimatsu, David A. Hart, Hidenori Otsubo, Kazunori Shimomura, Henning Madry, Tomoyuki Suzuki, Hideki Yoshikawa, Toshihiko Yamashita, Norimasa Nakamura

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Mesenchymal stem cell-(MSC-) based therapy is a promising treatment for cartilage. However, repair tissue in general fails to regenerate an original hyaline-like tissue. In this study, we focused on increasing the expression levels for insulin-like growth factor-1 (IGF-1) to improve repair tissue quality. The IGF-1 gene was introduced into human synovial MSCs with a lentiviral vector and examined the levels of gene expression and morphological status of MSCs under chondrogenic differentiation condition using pellet cultures. The size of the pellets derived from IGF-1-MSCs were significantly larger than those of the control group. The abundance of glycosaminoglycan (GAG) was also significantly higher in the IGF-1-MSC group. The histology of the IGF-1-induced pellets demonstrated similarities to hyaline cartilage without exhibiting features of a hypertrophic chondrocyte phenotype. Expression levels for the Col2A1 gene and protein were significantly higher in the IGF-1 pellets than in the control pellets, but expression levels for Col10, MMP-13, ALP, and Osterix were not higher. Thus, IGF-1 gene transfer to human synovial MSCs led to an improved chondrogenic differentiation capacity without the detectable induction of a hypertrophic or osteogenic phenotype.

Original languageEnglish
Article number5804147
JournalStem Cells International
Volume2017
DOIs
Publication statusPublished - 2017
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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