IGF-1 gene expression is differentially regulated by estrogen receptors α and β in mouse endometrial stromal cells and ovarian granulosa cells

Yuki Ogo, Shusuke Taniuchi, Fumiya Ojima, Sayo Hayashi, Itsuo Murakami, Yuka Saito, Sakae Takeuchi, Toshiyuki Kudo, Sumio Takahashi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Insulin-like growth factor 1 (IGF-1) is involved in regulations of reproductive functions in rats and mice. IGF- 1 expression is regulated by estrogen in several reproductive organs including the uterus and ovary. Two types of estrogen receptor (ERα and ERβ) are expressed in mouse uteri and ovaries, and it is unclear whether they differently mediate IGF- 1 gene transcription. To clarify the roles of ERα and ERβ, mouse endometrial stromal cells and ovarian granulosa cells were treated with ligands specific for individual estrogen receptors. In endometrial stromal cells, propyl-pyrazole-triol (PPT; ERα-selective agonist) increased Igf1 mRNA expression, which was suppressed by methyl-piperidino-pyrazole (MPP, ERα- selective antagonist), while diarylpropionitrile (DPN, ERβ-potency selective agonist) increased Igf1 mRNA expression, which was inhibited by MPP but not by 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-α]pyrimidin-3-yl]phenol (PHTPP; ERβ antagonist). PHTPP enhanced the DPN-induced increase in Igf1 mRNA expression. In ovarian granulosa cells, E2 and DPN decreased Igf1 mRNA expression, whereas PPT did not affect Igf1 mRNA levels. In these cells, PHTPP inhibited the DPN-induced decrease in Igf1 mRNA expression. These results suggest that ERα facilitates Igf1 transcription, whereas ERβ appears to inhibit Igf1 gene transcription in mouse endometrial stromal cells and ovarian granulosa cells.

Original languageEnglish
Pages (from-to)216-223
Number of pages8
JournalJournal of Reproduction and Development
Volume60
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Granulosa Cells
stromal cells
granulosa cells
somatomedins
Somatomedins
Stromal Cells
Estrogen Receptors
pyrazoles
Gene Expression
NAD
gene expression
Messenger RNA
transcription (genetics)
mice
uterus
agonists
antagonists
Insulin-Like Growth Factor I
Uterus
Ovary

Keywords

  • ERß
  • ERα
  • Insulin-like growth factor 1
  • Mouse

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Medicine(all)

Cite this

IGF-1 gene expression is differentially regulated by estrogen receptors α and β in mouse endometrial stromal cells and ovarian granulosa cells. / Ogo, Yuki; Taniuchi, Shusuke; Ojima, Fumiya; Hayashi, Sayo; Murakami, Itsuo; Saito, Yuka; Takeuchi, Sakae; Kudo, Toshiyuki; Takahashi, Sumio.

In: Journal of Reproduction and Development, Vol. 60, No. 3, 2014, p. 216-223.

Research output: Contribution to journalArticle

Ogo, Yuki ; Taniuchi, Shusuke ; Ojima, Fumiya ; Hayashi, Sayo ; Murakami, Itsuo ; Saito, Yuka ; Takeuchi, Sakae ; Kudo, Toshiyuki ; Takahashi, Sumio. / IGF-1 gene expression is differentially regulated by estrogen receptors α and β in mouse endometrial stromal cells and ovarian granulosa cells. In: Journal of Reproduction and Development. 2014 ; Vol. 60, No. 3. pp. 216-223.
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