IgE sensitivity to Malassezia pachydermatis and mite allergens in dogs with atopic dermatitis

Hironobu Ishimaru, Noriaki Okamoto, Masato Fujimura, Kazuki Miyaji, Hidekatsu Shimakura, Yukari Takase, Keijiro Mizukami, Jumpei Uchiyama, Douglas J. DeBoer, Masahiro Sakaguchi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In this study, dogs with atopic dermatitis were separated into non-food-induced atopic dermatitis (NFIAD) group (n = 15) and food-induced atopic dermatitis (FIAD) group (n = 37) based on an elimination diet test. IgE reactivity for crude Malassezia pachydermatis (M. pachydermatis) and house dust mites (HDM) allergen extracts was investigated in the two groups using fluorometric enzyme-linked immunosorbent assay (ELISA) and intradermal skin test (IDST). Nine (60%) of the 15 dogs in NFIAD group and 6 (16%) of the 37 dogs in FIAD group showed specific IgE for M. pachydermatis (Mann-Whitney U-test, P < 0.01). By immunoblotting analysis, the pooled serum samples from dogs with IgE for M. pachydermatis showed IgE reactivity for 50 kDa protein of M. pachydermatis. Twelve (80%) of the 15 dogs in NFIAD group and 8 (22%) of the 37 dogs in FIAD group showed specific IgE for HDM (Mann-Whitney U-test, P < 0.01). In addition, the dogs in NFIAD group significantly show a positive IDST to M. pachydermatis and HDM extracts compared with the dogs in FIAD group. The results suggest that dogs with NFIAD are at increased risk of becoming sensitized to the normal commensal organism M. pachydermatis compared with dogs with FIAD, perhaps co-sensitization occurred due to an HDM protease antigen's, Der f 1 and/or Der p 1, proteolytic activity related epidermal skin barrier defects. Treatment to limit skin colonization may thus be especially important in NFIAD.

Original languageEnglish
Article number110070
JournalVeterinary Immunology and Immunopathology
Volume226
DOIs
Publication statusPublished - Aug 2020
Externally publishedYes

Keywords

  • canine atopic dermatitis
  • co-sensitization
  • house dust mite
  • Malassezia pachydermatis
  • microbiome

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

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