IgE reactivity to fish allergens from Pacific cod (Gadus macrocephalus) in atopic dogs

Ichiro Imanishi, Jumpei Uchiyama, Keijiro Mizukami, Junichi Kamiie, Keigo Kurata, Keita Iyori, Masato Fujimura, Kuniyoshi Shimakura, Koji Nishifuji, Masahiro Sakaguchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Background: IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude extracts and purified allergens derived from the Pacific cod (Gadus macrocephalus) in atopic dogs to identify the allergenic proteins of cod. Results: The levels of specific IgE to crude cod extracts were measured in the sera of 179 atopic dogs, including 27 dogs with cod allergy, using enzyme-linked immunosorbent assay (ELISA). Specific IgE to crude cod extracts were present in 36 (20%) of the 179 atopic dogs and in 12 (44%) of the 27 dogs with cod allergy. The allergens in crude cod extracts were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that tested positive for specific IgE to crude cod extracts. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs tested positive for specific IgE to parvalbumin, collagen, and tropomyosin, respectively. Conclusions: The IgE reactivity to cod allergens observed in dogs was similar to that in humans, and this finding further supports the use of atopic dogs with fish allergy as a model for fish allergy in humans.

Original languageEnglish
Article number341
JournalBMC Veterinary Research
Volume16
Issue number1
DOIs
Publication statusPublished - Sep 16 2020
Externally publishedYes

Keywords

  • Allergen component
  • Animal model
  • Atopic dermatitis
  • Fish allergy
  • Immunoglobulin E

ASJC Scopus subject areas

  • veterinary(all)

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