Iejimalide C is a potent V-ATPase inhibitor, and induces actin disorganization

Sayaka Kazami, Masak Takaine, Hiroyasu Itoh, Takaaki Kubota, Jun'ichi Kobayashi, Takeo Usui

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Iejimalides (IEJLs) A-D are 24-membered macrolides isolated from a tunicate Eudistoma cf. rigida, and exhibit potent cytotoxicity in vitro and antitumor activity in vivo. We previously reported that the molecular target of IEJL-A and -B was the vacuolar-type H+-ATPases (V-ATPases). However IEJL-C and -D, which are sulfonylated IEJL-A and -B, respectively, show more potent antitumor activity, and their molecular targets remain to be discovered. Here, we report that IEJL-C is also a potent V-ATPase inhibitor by binding in a site similar to the bafilomycin-binding site. Two-hour treatment with IEJL-C resulted in the complete disappearance of acidic organelles in HeLa cells. Interestingly, after 24-h treatment, small actin aggregates were observed instead of actin fibers. The same actin reorganization was also observed in cells treated with another V-ATPase inhibitor, bafilomycin A1. Because IEJLs did not inhibit actin polymerization in vitro, these results suggest that the primary target of IEJL-C, as well as IEJL-A and -B, is V-ATPase, and actin reorganizations are probably caused by the disruption of pH homeostasis via V-ATPase inhibition.

Original languageEnglish
Pages (from-to)1944-1947
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume37
Issue number12
DOIs
Publication statusPublished - Dec 1 2014
Externally publishedYes

Keywords

  • Actin cytoskeleton
  • Antitumor
  • Cytotoxicity
  • Iejimalide C
  • V-ATPase

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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