Identification of Vanabin-interacting protein 1 (VIP1) from blood cells of the vanadium-rich ascidian Ascidia sydneiensis samea

Tatsuya Ueki, Koki Shintaku, Yuki Yonekawa, Nariaki Takatsu, Hiroshi Yamada, Toshiyuki Hamada, Hiroshi Hirota, Hitoshi Michibata

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Several species of ascidians, the so-called tunicates, accumulate extremely high levels of vanadium ions in their blood cells. We previously identified a family of vanadium-binding proteins, named Vanabins, from blood cells and blood plasma of a vanadium-rich ascidian, Ascidia sydneiensis samea. The 3-dimensional structure of Vanabin2, the predominant vanadium-binding protein in blood cells, has been revealed, and the vanadium-binding properties of Vanabin2 have been studied in detail. Here, we used Far Western blotting to identify a novel protein that interacts with Vanabin2 from a blood cell cDNA library. The protein, named Vanabin-interacting protein 1 (VIP1), was localized in the cytoplasm of signet ring cells and giant cells. Using a two-hybrid method, we revealed that VIP1 interacted with Vanabins 1, 2, 3, and 4 but not with Vanabin P. The N-terminal domain of VIP1 was shown to be important for the interaction. Further, Vanabin1 was found to interact with all of the other Vanabins. These results suggest that VIP1 and Vanabin1 act as metal chaperones or target proteins in vanadocytes.

Original languageEnglish
Pages (from-to)951-957
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume1770
Issue number6
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Urochordata
Vanadium
Blood Cells
Blood
Cells
Proteins
Carrier Proteins
Far-Western Blotting
Two-Hybrid System Techniques
Giant Cells
Gene Library
Cytoplasm
Metals
Ions
Plasmas

Keywords

  • Ascidian
  • Metal-binding protein
  • Protein-protein interaction
  • Vanadium

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of Vanabin-interacting protein 1 (VIP1) from blood cells of the vanadium-rich ascidian Ascidia sydneiensis samea. / Ueki, Tatsuya; Shintaku, Koki; Yonekawa, Yuki; Takatsu, Nariaki; Yamada, Hiroshi; Hamada, Toshiyuki; Hirota, Hiroshi; Michibata, Hitoshi.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1770, No. 6, 06.2007, p. 951-957.

Research output: Contribution to journalArticle

Ueki, Tatsuya ; Shintaku, Koki ; Yonekawa, Yuki ; Takatsu, Nariaki ; Yamada, Hiroshi ; Hamada, Toshiyuki ; Hirota, Hiroshi ; Michibata, Hitoshi. / Identification of Vanabin-interacting protein 1 (VIP1) from blood cells of the vanadium-rich ascidian Ascidia sydneiensis samea. In: Biochimica et Biophysica Acta - General Subjects. 2007 ; Vol. 1770, No. 6. pp. 951-957.
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