Identification of unique thyrotropin receptor (TSHR) splice variants in the chicken: The chicken TSHR gene revisited

Sylvia V H Grommen, Shusuke Taniuchi, Veerle M. Darras, Sumio Takahashi, Sakae Takeuchi, Bert De Groef

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We previously described the cloning of the full-length chicken thyrotropin receptor (TSHRa) and two splice variants, lacking exon 3 (TSHRb) or both exons 2 and 3 (TSHRc). Here we report the identification of three novel splice variants of the chicken TSHR, named TSHRd, -e and -f, differing in their C-terminal region and containing unique exonic sequences that are not present in the other TSHR variants. This finding suggests a TSHR gene structure with 13 rather than the previously assumed 10 exons. The three novel exons appear to be chicken-specific, as no equivalents of these exons were found in other vertebrate genomes. Like the full-length receptor, the five TSHR splice variants are most abundantly expressed in thyroid gland. TSHRb, -d, -e and -f mRNA was also present in virtually all extra-thyroidal tissues expressing TSHRa, whereas TSHRc shows a more restricted tissue distribution. Whether these receptor transcripts are translated to functional proteins needs to be verified, but if so, they could be attributed various physiological roles.

Original languageEnglish
Pages (from-to)460-463
Number of pages4
JournalGeneral and Comparative Endocrinology
Volume156
Issue number3
DOIs
Publication statusPublished - May 1 2008

Fingerprint

Thyrotropin Receptors
exons
Chickens
Exons
chickens
Genes
genes
receptors
thyroid gland
tissue distribution
Tissue Distribution
Vertebrates
Organism Cloning
molecular cloning
Thyroid Gland
vertebrates
thyrotropin receptors
Genome
Messenger RNA
genome

Keywords

  • Alternative splicing
  • Chicken
  • Gene structure
  • Thyrotropin receptor

ASJC Scopus subject areas

  • Endocrinology

Cite this

Identification of unique thyrotropin receptor (TSHR) splice variants in the chicken : The chicken TSHR gene revisited. / Grommen, Sylvia V H; Taniuchi, Shusuke; Darras, Veerle M.; Takahashi, Sumio; Takeuchi, Sakae; Groef, Bert De.

In: General and Comparative Endocrinology, Vol. 156, No. 3, 01.05.2008, p. 460-463.

Research output: Contribution to journalArticle

@article{fe340708d55840c8996d120e26f8b3ea,
title = "Identification of unique thyrotropin receptor (TSHR) splice variants in the chicken: The chicken TSHR gene revisited",
abstract = "We previously described the cloning of the full-length chicken thyrotropin receptor (TSHRa) and two splice variants, lacking exon 3 (TSHRb) or both exons 2 and 3 (TSHRc). Here we report the identification of three novel splice variants of the chicken TSHR, named TSHRd, -e and -f, differing in their C-terminal region and containing unique exonic sequences that are not present in the other TSHR variants. This finding suggests a TSHR gene structure with 13 rather than the previously assumed 10 exons. The three novel exons appear to be chicken-specific, as no equivalents of these exons were found in other vertebrate genomes. Like the full-length receptor, the five TSHR splice variants are most abundantly expressed in thyroid gland. TSHRb, -d, -e and -f mRNA was also present in virtually all extra-thyroidal tissues expressing TSHRa, whereas TSHRc shows a more restricted tissue distribution. Whether these receptor transcripts are translated to functional proteins needs to be verified, but if so, they could be attributed various physiological roles.",
keywords = "Alternative splicing, Chicken, Gene structure, Thyrotropin receptor",
author = "Grommen, {Sylvia V H} and Shusuke Taniuchi and Darras, {Veerle M.} and Sumio Takahashi and Sakae Takeuchi and Groef, {Bert De}",
year = "2008",
month = "5",
day = "1",
doi = "10.1016/j.ygcen.2008.03.003",
language = "English",
volume = "156",
pages = "460--463",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Identification of unique thyrotropin receptor (TSHR) splice variants in the chicken

T2 - The chicken TSHR gene revisited

AU - Grommen, Sylvia V H

AU - Taniuchi, Shusuke

AU - Darras, Veerle M.

AU - Takahashi, Sumio

AU - Takeuchi, Sakae

AU - Groef, Bert De

PY - 2008/5/1

Y1 - 2008/5/1

N2 - We previously described the cloning of the full-length chicken thyrotropin receptor (TSHRa) and two splice variants, lacking exon 3 (TSHRb) or both exons 2 and 3 (TSHRc). Here we report the identification of three novel splice variants of the chicken TSHR, named TSHRd, -e and -f, differing in their C-terminal region and containing unique exonic sequences that are not present in the other TSHR variants. This finding suggests a TSHR gene structure with 13 rather than the previously assumed 10 exons. The three novel exons appear to be chicken-specific, as no equivalents of these exons were found in other vertebrate genomes. Like the full-length receptor, the five TSHR splice variants are most abundantly expressed in thyroid gland. TSHRb, -d, -e and -f mRNA was also present in virtually all extra-thyroidal tissues expressing TSHRa, whereas TSHRc shows a more restricted tissue distribution. Whether these receptor transcripts are translated to functional proteins needs to be verified, but if so, they could be attributed various physiological roles.

AB - We previously described the cloning of the full-length chicken thyrotropin receptor (TSHRa) and two splice variants, lacking exon 3 (TSHRb) or both exons 2 and 3 (TSHRc). Here we report the identification of three novel splice variants of the chicken TSHR, named TSHRd, -e and -f, differing in their C-terminal region and containing unique exonic sequences that are not present in the other TSHR variants. This finding suggests a TSHR gene structure with 13 rather than the previously assumed 10 exons. The three novel exons appear to be chicken-specific, as no equivalents of these exons were found in other vertebrate genomes. Like the full-length receptor, the five TSHR splice variants are most abundantly expressed in thyroid gland. TSHRb, -d, -e and -f mRNA was also present in virtually all extra-thyroidal tissues expressing TSHRa, whereas TSHRc shows a more restricted tissue distribution. Whether these receptor transcripts are translated to functional proteins needs to be verified, but if so, they could be attributed various physiological roles.

KW - Alternative splicing

KW - Chicken

KW - Gene structure

KW - Thyrotropin receptor

UR - http://www.scopus.com/inward/record.url?scp=42649096022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42649096022&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2008.03.003

DO - 10.1016/j.ygcen.2008.03.003

M3 - Article

C2 - 18400223

AN - SCOPUS:42649096022

VL - 156

SP - 460

EP - 463

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 3

ER -