Identification of two types of growth hormone receptor mutations in two strains of sex-linked dwarf chickens

Kenichi Tahara, Akira Tsukada, Takanobu Hanai, Kenta Okumura, Kikumi Yamada, Atsushi Murai, Rikiya Yamamoto, Makoto Maeno, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sex-linked dwarf (SLD) of White Leghorn (WL; S23MA line, Okazaki station, Japan) and White Plymouth Rock (WPR; 15 line, Hyogo station, Japan) strains were established from the MA line (WL) and 16 line (WPR) of a normal growing line, respectively. However, the responsible genes in the two lines of SLD chicken have not been identified. In this study, we characterized the phenotypes and identified the responsible genes in the SLD chickens of these two strains. SLD chickens of both strains showed low body weight, short tarsometatarsus length, and high blood growth hormone (GH) level compared with the normal growing lines. From these particular results, it was indicated that the SLD chickens might possess a defect in the growth hormone receptor (GHR). We identified two types of mutations in the GHR gene in each SLD chicken by Northern blot and polymerase chain reaction analyses. The S23MA line had a single base mutation in the splice donor site of the exon 5/intron 5 on the GHR gene, whereas the 15 line lacked a large part of exon 10 of the GHR gene, which contained 27 highly conserved amino acids at the 3' end of the coding region and 3'-UTR. Furthermore, it was revealed that growth retardation was caused by reduction in food intake of the SLD chickens. These two genetically distinguishable lines of dwarf chickens would serve as an effective tool for analyzing novel GH function and GHR signal transduction in chickens.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
JournalJournal of Poultry Science
Volume46
Issue number3
DOIs
Publication statusPublished - Jul 25 2009
Externally publishedYes

Fingerprint

chickens
mutation
gender
sex linkage
somatotropin
exons
Plymouth Rock
Japan
genes
somatotropin receptors
3' untranslated regions
White Leghorn
Northern blotting
growth retardation
signal transduction
introns
food intake
polymerase chain reaction
phenotype
amino acids

Keywords

  • Genotyping
  • Growth hormone
  • Growth hormone receptor
  • Growth hormone resistance
  • Sex-linked dwarf chicken

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Tahara, K., Tsukada, A., Hanai, T., Okumura, K., Yamada, K., Murai, A., ... Shimada, K. (2009). Identification of two types of growth hormone receptor mutations in two strains of sex-linked dwarf chickens. Journal of Poultry Science, 46(3), 249-256. https://doi.org/10.2141/jpsa.46.249

Identification of two types of growth hormone receptor mutations in two strains of sex-linked dwarf chickens. / Tahara, Kenichi; Tsukada, Akira; Hanai, Takanobu; Okumura, Kenta; Yamada, Kikumi; Murai, Atsushi; Yamamoto, Rikiya; Maeno, Makoto; Saito, Noboru; Shimada, Kiyoshi.

In: Journal of Poultry Science, Vol. 46, No. 3, 25.07.2009, p. 249-256.

Research output: Contribution to journalArticle

Tahara, K, Tsukada, A, Hanai, T, Okumura, K, Yamada, K, Murai, A, Yamamoto, R, Maeno, M, Saito, N & Shimada, K 2009, 'Identification of two types of growth hormone receptor mutations in two strains of sex-linked dwarf chickens', Journal of Poultry Science, vol. 46, no. 3, pp. 249-256. https://doi.org/10.2141/jpsa.46.249
Tahara, Kenichi ; Tsukada, Akira ; Hanai, Takanobu ; Okumura, Kenta ; Yamada, Kikumi ; Murai, Atsushi ; Yamamoto, Rikiya ; Maeno, Makoto ; Saito, Noboru ; Shimada, Kiyoshi. / Identification of two types of growth hormone receptor mutations in two strains of sex-linked dwarf chickens. In: Journal of Poultry Science. 2009 ; Vol. 46, No. 3. pp. 249-256.
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