Identification of two subtypes of protein kinase C in human placenta

Seiji Nomura; Hiroshi Tokumitsu; Shigehiko Mizutani; Osamu Narita; Yutaka Tomada; Hiroyoshi Hidaka

doi:10.1016/0143-4004(91)90495-2

Identification of two subtypes of protein kinase C in human placenta

Seiji Nomura, Hiroshi Tokumitsu, Shigehiko Mizutani, Osamu Narita, Yutaka Tomada, Hiroyoshi Hidaka

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A purified protein kinase C (PKC) has been isolated from term human placental tissue, which is phospholipid and Ca²⁺-dependent. Two subtypes of the enzyme were identified by hydroxyapatite column chromatography and using monoclonal antibodies with immunohistochemical techniques; subtype III is present in higher concentration than subtype II. Their ratio of 2.5 is very similar in second and third trimester placentas, but is higher, 6.5, in the first trimester. The subtype I was never expressed.

Original language	English
Pages (from-to)	605-613
Number of pages	9
Journal	Placenta
Volume	12
Issue number	6
DOIs	https://doi.org/10.1016/0143-4004(91)90495-2
Publication status	Published - Jan 1 1991
Externally published	Yes

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynaecology
Developmental Biology

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10.1016/0143-4004(91)90495-2

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T1 - Identification of two subtypes of protein kinase C in human placenta

AU - Nomura, Seiji

AU - Tokumitsu, Hiroshi

AU - Mizutani, Shigehiko

AU - Narita, Osamu

AU - Tomada, Yutaka

AU - Hidaka, Hiroyoshi

PY - 1991/1/1

Y1 - 1991/1/1

N2 - A purified protein kinase C (PKC) has been isolated from term human placental tissue, which is phospholipid and Ca2+-dependent. Two subtypes of the enzyme were identified by hydroxyapatite column chromatography and using monoclonal antibodies with immunohistochemical techniques; subtype III is present in higher concentration than subtype II. Their ratio of 2.5 is very similar in second and third trimester placentas, but is higher, 6.5, in the first trimester. The subtype I was never expressed.

AB - A purified protein kinase C (PKC) has been isolated from term human placental tissue, which is phospholipid and Ca2+-dependent. Two subtypes of the enzyme were identified by hydroxyapatite column chromatography and using monoclonal antibodies with immunohistochemical techniques; subtype III is present in higher concentration than subtype II. Their ratio of 2.5 is very similar in second and third trimester placentas, but is higher, 6.5, in the first trimester. The subtype I was never expressed.

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Identification of two subtypes of protein kinase C in human placenta

Abstract

ASJC Scopus subject areas

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